Dynamic Modeling and Metabolic Flux Analysis for Optimized Production of Rhamnolipids

A comprehensive metabolic network based on the fundamental pathways representing the central metabolism of rhamnolipid by Pseudomonas aeruginosa is proposed and a dynamic model compatible with the underlying metabolic network is developed involving the macro-reactions derived from the elementary flu...

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Bibliographic Details
Published in:Chemical engineering communications 2016-03, Vol.203 (3), p.326-338
Main Authors: Satya Eswari, J., Venkateswarlu, Ch
Format: Article
Language:English
Subjects:
Bioprocess
Dynamic model
Dynamic models
Evolutionary
Evolutionary optimization
Flux
Flux analysis
Fluxes
Global optimization
Lipids
Metabolic network
Metabolism
Metabolites
Networks
Optimization
Optimization techniques
Pseudomonas aeruginosa
Rhamnolipids
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Summary:A comprehensive metabolic network based on the fundamental pathways representing the central metabolism of rhamnolipid by Pseudomonas aeruginosa is proposed and a dynamic model compatible with the underlying metabolic network is developed involving the macro-reactions derived from the elementary flux modes of the reaction network. The experimentally validated mathematical model is then coupled with a global optimization technique called differential evolution (DE) to optimize the medium composition as well as the extracellular and intracellular fluxes of the metabolic network. The analysis of the results shows the usefulness of the integrated approach involving the development of a dynamic model based on the metabolic network structure and model-based optimization of the medium composition and metabolic fluxes by an efficient evolutionary optimization technique to enhance the productivity of rhamnolipid.
ISSN:0098-6445
1563-5201
DOI:10.1080/00986445.2014.996638