Effect of Conjugated Linoleic Acids (CLA) Supplementation on the Activity of Enzymes Participating in the Formation of Arachidonic Acid in Liver Microsomes of Rats—Probable Mechanism of CLA Anticancer Activity

• Published in: Nutrition and cancer 2015-01, Vol.67 (1), p.145-155
• Main Authors: Stawarska, Agnieszka, Białek, Agnieszka, Stanimirova, Ivana, Stawarski, Tomasz, Tokarz, Andrzej
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Adenocarcinoma - prevention & control; Animals; anticarcinogenic activity; Anticarcinogenic Agents - administration & dosage; Anticarcinogenic Agents - therapeutic use; arachidonic acid; Arachidonic Acid - metabolism; carcinogenicity; Chromatography; conjugated linoleic acid; diet; Dietary Supplements; Effects; enzyme activity; Enzymes; Fatty Acid Desaturases - chemistry; Fatty Acid Desaturases - metabolism; Fatty acids; isomers; Linoleic Acids, Conjugated - administration & dosage; Linoleic Acids, Conjugated - therapeutic use; liquid chromatography; Liver; Liver - enzymology; Liver - metabolism; Liver - pathology; liver microsomes; Male; Mammary Neoplasms, Experimental - metabolism; Mammary Neoplasms, Experimental - pathology; Mammary Neoplasms, Experimental - prevention & control; Microsomes, Liver - enzymology; Microsomes, Liver - metabolism; Organ Size; Random Allocation; rats; Rats, Sprague-Dawley; Rodents; Stearoyl-CoA Desaturase - chemistry; Stearoyl-CoA Desaturase - metabolism; Survival Analysis; Tumor Burden
• ISSN: 1532-7914; 0163-5581; 1532-7914
• DOI: 10.1080/01635581.2015.967875

The aim of the present research was to examine the effect of conjugated linoleic acids (CLA) supplementation on the activity of enzymes that take part in the synthesis of arachidonic acid (AA) and also to investigate the relation between their activity and the neoplastic process. The enzyme activities were established indirectly, because their measure was the amount of AA formed in vitro, being developed from linoleic acid as determined in liver microsomes of Spraque-Dawley rats. In addition, the indices of Δ ⁶-desaturase (D6D) and Δ ⁵-desaturase (D5D) were determined. To this aim, the method of high per-formance liquid chromatography with UV/VIS detection was used. Between the examined groups, statistically significant differences were observed in the activities of enzymes as well as D6D. The carcinogenic agent applied (DMBA) was found to significantly increase the activity of the examined enzymes. Negative correlation was found between the activities of desaturases and CLA supplementation, whereas the activity of those enzymes was a little higher in the group of rats with chemically induced cancer process. The neoplastic process has a stimulating effect on the activity of D6D. The decrease of D6D activity, resulting from the presence of CLA in the animals’ diet, may confirm the anticancer properties of these isomers.