Low phosphate nutrition alters bean plants' ability to assimilate and translocate nitrate

• Published in: Journal of plant nutrition 1999, Vol.22 (3), p.551-563
• Main Authors: Gniazdowska, A, Krawczak, A, Mikulska, M, Rychter, A.M
• Format: Article
• Language: English
• Subjects: Absorption. Translocation of ions and substances. Permeability; Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; enzyme activity; Fundamental and applied biological sciences. Psychology; leaves; nitrate reductase; nitrates; nitrogen content; nutrient content; nutrient solutions; nutrient transport; nutrient uptake; nutrient-nutrient interactions; Nutrition. Photosynthesis. Respiration. Metabolism; Phaseolus vulgaris; phosphates; roots; shoots
• ISSN: 0190-4167; 1532-4087
• DOI: 10.1080/01904169909365651

Bean plants (Phaseolus vulgaris L.) were cultured for 10 or 18 days on phosphate sufficient (+P) or phosphate deficient (-P) nutrient medium. Nitrate and phosphate distribution between shoot and root, nitrate uptake, and nitrate reductase activity (NR activity, in vivo and in vitro) in root and leaves was estimated. The decrease in Pi concentration in leaves and roots led to decreased rate of NO3 uptake and increased NO3 accumulation in roots, accompanied by alterations in NO3 distribution between shoot and roots. Nitrate reductase activity estimated in vitro was twice higher than estimated in vivo and both in +P and -P plants was lower in the roots than in the shoots. The decrease of NR activity in -P plants was more pronounced in the roots and after 2 weeks of phosphate starvation it was about 40% lower as compared with the control. The depression in nitrate uptake may be the result of feedback inhibition due to accumulation of nitrate in the roots. The increased NO3 concentration in root tissue may be explained by decreased NR activity and lower transport of nitrate from roots to shoot.