Molecular cloning, expression and characterization of bile salt hydrolase from Lactobacillus rhamnosus E9 strain

Bile salt hydrolase (BSH) enzyme, commonly found in probiotic bacteria of gut origin, catalyzes the hydrolysis of glycine and/or taurine-conjugated bile salts allowing for colonization of the bacteria in the gut and contributing to a decrease in levels of cholesterol. However an excessive deconjugat...

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Bibliographic Details
Published in:Food biotechnology 2017-04, Vol.31 (2), p.128-140
Main Authors: Kaya, Yeşim, Kök, M. Şamil, Öztürk, Mehmet
Format: Article
Language:English
Subjects:
Amino acids
Bacteria
Bile
Bile acids
bile salt hydrolase
Bile salts
Cholesterol
Cloning
Colonization
conjugated bile salts
E coli
enzyme processing
Enzymes
Glycine
Hydrolase
Hydrolysis
Lactic acid bacteria
Lactobacilli
Lactobacillus rhamnosus
Molecular weight
Nucleotide sequence
Nucleotides
Probiotics
Salt
Salts
substrate specificity
Taurine
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Summary:Bile salt hydrolase (BSH) enzyme, commonly found in probiotic bacteria of gut origin, catalyzes the hydrolysis of glycine and/or taurine-conjugated bile salts allowing for colonization of the bacteria in the gut and contributing to a decrease in levels of cholesterol. However an excessive deconjugation of tauro-conjugated bile salts and production of secondary bile acid can have harmful side-effects. The aim of this study was to characterize the activity of BSH enzymes from Lactobacillus rhamnosus E9, a popular probiotic strain. The bsh gene was cloned, expressed, purified and characterized in Escherichia coli BLR(DE3) strain. The hydrolysis activities and substrate specificities of the recombinant BSH (rBSH) enzyme were examined using six different bile acids. Nucleotide sequence analysis results indicated that the bsh of E9 contained an open reading frame (ORF) of 1014 and nucleotides encoding a 338-amino acid protein with a molecular weight of 37 kDa. Five catalytically important amino acids and the amino acid motifs located around the active site were highly conserved. The rBSH showed a slight preference towards glycine-conjugated to tauro-conjugated bile salts. This confirms that it is a safe strain for probiotics and its preference for glycine-conjugated bile salts should be further investigated.
ISSN:0890-5436
1532-4249
DOI:10.1080/08905436.2017.1303778