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Designed and fabrication of triple-layered vascular scaffold with microchannels
Currently, one of the best preparation strategies for the triple-layered vascular scaffold is to imitate the three-layer structure of natural blood vessels to achieve the biofunctional characteristics of vascular transplantation. Here, we developed a combinatorial method to fabricate triple-layered...
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Published in: | Journal of biomaterials science. Polymer ed. 2021-04, Vol.32 (6), p.714-734 |
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container_title | Journal of biomaterials science. Polymer ed. |
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creator | Hu, Qingxi Shen, Zhipeng Zhang, Haiguang Liu, Suihong Feng, Rui Feng, Jiaxuan Ramalingam, Murugan |
description | Currently, one of the best preparation strategies for the triple-layered vascular scaffold is to imitate the three-layer structure of natural blood vessels to achieve the biofunctional characteristics of vascular transplantation. Here, we developed a combinatorial method to fabricate triple-layered vascular scaffold (TVS) by using electrospinning and coaxial 3 D printing. First, Polycaprolactone-collagen (PCL-Col) was applied to prepared the inner layer of TVS by electrospinning. Second, egg white/sodium alginate (EW/SA) blend hydrogel was extruded to form hollow filaments by coaxial 3 D printing and crosslinking mechanism, which enwound around the surface of the inner layer in a circumferential direction as the intermediate layer of TVS. Finally, electrospun PCL-Col nanofibers were wrapped on the surface of hydrogel layer as the outer layer of TVS. The morphological characterization and mechanical strength of the fabricated TVS were measured. Compared with natural blood vessels, results shown that ultimate tensile stress (UTS), strain to failure (STF), the estimated burst strength and the suture retention strength (SRS) of TVS were superior. Also, the fabricated TVS exhibits good hydrophilicity and excellent flexibility. Moreover, the biocompatibility of TVS was investigated through human umbilical vein endothelial cells (HUVECs), the results demonstrated that cells can successfully attach the surface of graft and maintain high viability. In summary, all of results demonstrated that this method could fabricate a novel triple-layered vascular scaffold, possessing appropriate mechanical properties and good biological properties, which has the potential to be used in tissue engineered vascular grafts applications. |
doi_str_mv | 10.1080/09205063.2020.1864083 |
format | article |
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Here, we developed a combinatorial method to fabricate triple-layered vascular scaffold (TVS) by using electrospinning and coaxial 3 D printing. First, Polycaprolactone-collagen (PCL-Col) was applied to prepared the inner layer of TVS by electrospinning. Second, egg white/sodium alginate (EW/SA) blend hydrogel was extruded to form hollow filaments by coaxial 3 D printing and crosslinking mechanism, which enwound around the surface of the inner layer in a circumferential direction as the intermediate layer of TVS. Finally, electrospun PCL-Col nanofibers were wrapped on the surface of hydrogel layer as the outer layer of TVS. The morphological characterization and mechanical strength of the fabricated TVS were measured. Compared with natural blood vessels, results shown that ultimate tensile stress (UTS), strain to failure (STF), the estimated burst strength and the suture retention strength (SRS) of TVS were superior. Also, the fabricated TVS exhibits good hydrophilicity and excellent flexibility. Moreover, the biocompatibility of TVS was investigated through human umbilical vein endothelial cells (HUVECs), the results demonstrated that cells can successfully attach the surface of graft and maintain high viability. In summary, all of results demonstrated that this method could fabricate a novel triple-layered vascular scaffold, possessing appropriate mechanical properties and good biological properties, which has the potential to be used in tissue engineered vascular grafts applications.</description><identifier>ISSN: 0920-5063</identifier><identifier>EISSN: 1568-5624</identifier><identifier>DOI: 10.1080/09205063.2020.1864083</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Coaxial 3D printing ; Electrospinning ; EW-SA Hydrogel ; Hydrogels ; PCL-Col ; Triple-layered vascular scaffold</subject><ispartof>Journal of biomaterials science. 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Polymer ed.</title><description>Currently, one of the best preparation strategies for the triple-layered vascular scaffold is to imitate the three-layer structure of natural blood vessels to achieve the biofunctional characteristics of vascular transplantation. Here, we developed a combinatorial method to fabricate triple-layered vascular scaffold (TVS) by using electrospinning and coaxial 3 D printing. First, Polycaprolactone-collagen (PCL-Col) was applied to prepared the inner layer of TVS by electrospinning. Second, egg white/sodium alginate (EW/SA) blend hydrogel was extruded to form hollow filaments by coaxial 3 D printing and crosslinking mechanism, which enwound around the surface of the inner layer in a circumferential direction as the intermediate layer of TVS. Finally, electrospun PCL-Col nanofibers were wrapped on the surface of hydrogel layer as the outer layer of TVS. The morphological characterization and mechanical strength of the fabricated TVS were measured. Compared with natural blood vessels, results shown that ultimate tensile stress (UTS), strain to failure (STF), the estimated burst strength and the suture retention strength (SRS) of TVS were superior. Also, the fabricated TVS exhibits good hydrophilicity and excellent flexibility. Moreover, the biocompatibility of TVS was investigated through human umbilical vein endothelial cells (HUVECs), the results demonstrated that cells can successfully attach the surface of graft and maintain high viability. In summary, all of results demonstrated that this method could fabricate a novel triple-layered vascular scaffold, possessing appropriate mechanical properties and good biological properties, which has the potential to be used in tissue engineered vascular grafts applications.</description><subject>Coaxial 3D printing</subject><subject>Electrospinning</subject><subject>EW-SA Hydrogel</subject><subject>Hydrogels</subject><subject>PCL-Col</subject><subject>Triple-layered vascular scaffold</subject><issn>0920-5063</issn><issn>1568-5624</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kFtLAzEQhYMoWKs_QVjweWsum728KfUKhb7oc5jNxaakSU22lv57s7S--jIzDN85MxyEbgmeEdzie9xRzHHNZhTTvGrrCrfsDE0Ir9uS17Q6R5ORKUfoEl2ltMYYE0zYBC2fdLJfXqsCvCoM9NFKGGzwRTDFEO3W6dLBQcdM_ECSOwexSBKMCU4Vezusio2VMcgVeK9dukYXBlzSN6c-RZ8vzx_zt3KxfH2fPy5KWfFmKHvZKSCkh6ZWsmpqovumzQOjuqaG014xpnvoKOt1wxRrJJctBY0bo7SWHZuiu6PvNobvnU6DWIdd9PmkoJxQ3uJcM8WPVP4wpaiN2Ea7gXgQBIsxO_GXnRizE6fssu7hqLPehLiBfYhOiQEOLkQTwUubBPvf4heOxnbJ</recordid><startdate>20210413</startdate><enddate>20210413</enddate><creator>Hu, Qingxi</creator><creator>Shen, Zhipeng</creator><creator>Zhang, Haiguang</creator><creator>Liu, Suihong</creator><creator>Feng, Rui</creator><creator>Feng, Jiaxuan</creator><creator>Ramalingam, Murugan</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>20210413</creationdate><title>Designed and fabrication of triple-layered vascular scaffold with microchannels</title><author>Hu, Qingxi ; Shen, Zhipeng ; Zhang, Haiguang ; Liu, Suihong ; Feng, Rui ; Feng, Jiaxuan ; Ramalingam, Murugan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-bc9da11ba76dc4761eb78c4732e62f52bd33eba923be73d37c5c82ae07fdeec93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Coaxial 3D printing</topic><topic>Electrospinning</topic><topic>EW-SA Hydrogel</topic><topic>Hydrogels</topic><topic>PCL-Col</topic><topic>Triple-layered vascular scaffold</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Qingxi</creatorcontrib><creatorcontrib>Shen, Zhipeng</creatorcontrib><creatorcontrib>Zhang, Haiguang</creatorcontrib><creatorcontrib>Liu, Suihong</creatorcontrib><creatorcontrib>Feng, Rui</creatorcontrib><creatorcontrib>Feng, Jiaxuan</creatorcontrib><creatorcontrib>Ramalingam, Murugan</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Journal of biomaterials science. Polymer ed.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Qingxi</au><au>Shen, Zhipeng</au><au>Zhang, Haiguang</au><au>Liu, Suihong</au><au>Feng, Rui</au><au>Feng, Jiaxuan</au><au>Ramalingam, Murugan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Designed and fabrication of triple-layered vascular scaffold with microchannels</atitle><jtitle>Journal of biomaterials science. Polymer ed.</jtitle><date>2021-04-13</date><risdate>2021</risdate><volume>32</volume><issue>6</issue><spage>714</spage><epage>734</epage><pages>714-734</pages><issn>0920-5063</issn><eissn>1568-5624</eissn><abstract>Currently, one of the best preparation strategies for the triple-layered vascular scaffold is to imitate the three-layer structure of natural blood vessels to achieve the biofunctional characteristics of vascular transplantation. Here, we developed a combinatorial method to fabricate triple-layered vascular scaffold (TVS) by using electrospinning and coaxial 3 D printing. First, Polycaprolactone-collagen (PCL-Col) was applied to prepared the inner layer of TVS by electrospinning. Second, egg white/sodium alginate (EW/SA) blend hydrogel was extruded to form hollow filaments by coaxial 3 D printing and crosslinking mechanism, which enwound around the surface of the inner layer in a circumferential direction as the intermediate layer of TVS. Finally, electrospun PCL-Col nanofibers were wrapped on the surface of hydrogel layer as the outer layer of TVS. The morphological characterization and mechanical strength of the fabricated TVS were measured. Compared with natural blood vessels, results shown that ultimate tensile stress (UTS), strain to failure (STF), the estimated burst strength and the suture retention strength (SRS) of TVS were superior. Also, the fabricated TVS exhibits good hydrophilicity and excellent flexibility. Moreover, the biocompatibility of TVS was investigated through human umbilical vein endothelial cells (HUVECs), the results demonstrated that cells can successfully attach the surface of graft and maintain high viability. In summary, all of results demonstrated that this method could fabricate a novel triple-layered vascular scaffold, possessing appropriate mechanical properties and good biological properties, which has the potential to be used in tissue engineered vascular grafts applications.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><doi>10.1080/09205063.2020.1864083</doi><tpages>21</tpages></addata></record> |
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subjects | Coaxial 3D printing Electrospinning EW-SA Hydrogel Hydrogels PCL-Col Triple-layered vascular scaffold |
title | Designed and fabrication of triple-layered vascular scaffold with microchannels |
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