Improved insecticidal activity of a genetically modified baculovirus expressing the immunosuppressive CrV1 protein from a polydnavirus against Spodoptera exigua

Recombinant baculoviruses could be used as biological insecticides through the introduction and expression of exogenous genes (such as those coding for proteins) that interfere with metabolism, metamorphosis (toxins, hormones, and enzymes), and immune system of the insects. The CrV1 secreted protein...

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Bibliographic Details
Published in:Biocontrol science and technology 2016-01, Vol.26 (1), p.1-11
Main Authors: Wei, Lihua, Pérez-Rodríguez, Miguel Á., Tamez-Guerra, Patricia, De Luna-Santillana, Erick de Jesús, Rosas-García, Ninfa M., Villegas-Mendoza, Jesús M., Rodríguez-Pérez, Mario A.
Format: Article
Language:English
Subjects:
Autographa californica
Baculoviridae
Baculovirus
baculoviruses
Cotesia rubecula
CrV1 protein
Hymenoptera
Nuclear polyhedrosis virus
Polydnavirus
Spodoptera exigua
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Summary:Recombinant baculoviruses could be used as biological insecticides through the introduction and expression of exogenous genes (such as those coding for proteins) that interfere with metabolism, metamorphosis (toxins, hormones, and enzymes), and immune system of the insects. The CrV1 secreted protein of Cotesia rubecula polydnavirus (PDV) is responsible for the actin depolymerisation in haemocytes and the abolishment of immune functions such as phagocytosis and cell spreading, thus allowing the successful embryonic development of the parasitoid wasp. CrV1 cDNA was cloned into C6 strain of Autographa californica multiple nucleopolyhedrovirus (AcMNPV-C6-CrV1) under p10 promoter to construct a recombinant virus. The recombinant virus was then tested against the insect pest Spodoptera exigua. The recombinant virus expressing CrV1 protein showed significantly lower LC 50 and shorter LT 50 as compared with the AcMNPV-C6 wild-type virus. The potential of recombinant baculoviruses expressing PDV genes in relation to their virulence is discussed.
ISSN:0958-3157
1360-0478
DOI:10.1080/09583157.2015.1072129