Validated Capillary Electrophoresis Study for the Determination of Cetirizine in Pharmaceutical Forms

A capillary electrophoretic method for the determination of cetirizine is described in this study. The method was developed by using a running buffer consisting of 10 mM of 10% methanol with pH 8.5, employing a fused silica column with a total length of 85 cm, an effective length of 65 cm, and inter...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2006-08, Vol.29 (12), p.1781-1792
Main Authors: UYSAL, Ülkü Dilek, TUNNEL, Muzaffer
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Capillary electrophoresis
Cetirizine
Determination
General pharmacology
Medical sciences
Method development
Method validation
Pharmaceutical analysis
Pharmacology. Drug treatments
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Summary:A capillary electrophoretic method for the determination of cetirizine is described in this study. The method was developed by using a running buffer consisting of 10 mM of 10% methanol with pH 8.5, employing a fused silica column with a total length of 85 cm, an effective length of 65 cm, and internal diameter of 75 µm. 28 kV were applied, which produced signals that were detected at 200 nm. Under these conditions, cetirizine and phenobarbital sodium as an internal standard appeared at 6.7 and 8.9 minutes, respectively. The limits of detection and quantification were found to be 5.45×10 −6  M and 1.60×10 −5  M, respectively. The repeatability and linearity of the method were validated by intra-day and inter-day precision. Then, the proposed method was applied to Zyrtec ® tablet, syrup, and oral drop. The results indicate that the method is simple, accurate, and precise for the analysis of cetirizine.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826070600716983