Development of a Method to Evaluate the Release Profile of Tamoxifen from Pegylated Hybrid Micelles

Studies of in vitro release profiles are important during the development of drug delivery systems. This article describes the development and validation of a RP-HPLC method for the quantification of drug released from tamoxifen-loaded DSPE-PEG(2000)/TPGS micelles and the determination of the best d...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2015-07, Vol.38 (12), p.1223-1229
Main Authors: Claro de Souza, Marina, Marotta-Oliveira, Samantha Sant'Anna, Rocha, Nathália Helena Souza, Eloy, Josimar O., Marchetti, Juliana Maldonado
Format: Article
Language:English
Subjects:
analytical development and validation
drug delivery systems
hybrid pegylated micelles
in vitro release profile
RP-HPLC quantification
tamoxifen
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Summary:Studies of in vitro release profiles are important during the development of drug delivery systems. This article describes the development and validation of a RP-HPLC method for the quantification of drug released from tamoxifen-loaded DSPE-PEG(2000)/TPGS micelles and the determination of the best dissolution medium and apparatus for this assay. The method showed specificity, linearity, precision, accuracy, and robustness for the parameters evaluated (range 0.25-20.0 µg mL −1 ). The best dissolution medium was 1.0 mol L −1 sodium salicylate, pH 7.4, and the most suitable apparatus was a PVC tube with one end covered with a dialysis membrane. This method was shown to be useful for tamoxifen quantification in in vitro release studies.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2015.1032418