Validation of SNP marker linked to alc gene for long shelf life of tomato

Tomato (Solanum lycopersicum L.) fruits are naturally perishable and have short shelf life. Post-harvest losses can be devastating and can be associated with rapid ripening. The objective of this study was to identify a single nucleotide polymorphism (SNP) marker associated with alc gene conferring...

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Bibliographic Details
Published in:Journal of crop improvement 2019-09, Vol.33 (5), p.669-682
Main Authors: Osei, M.K., Danquah, E., Danquah, A., Massoudi, M., Maxwell, D., Adu-Dapaah, H., Blay, E.
Format: Article
Language:English
Subjects:
Alc gene
Fruits
Gene polymorphism
Goodness of fit
Loci
mutant
Nucleotides
Polymorphism
Post-harvest decay
Ripening
Shelf life
Single-nucleotide polymorphism
SNP marker
Solanum lycopersicum
Statistical tests
tomato
Tomatoes
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Summary:Tomato (Solanum lycopersicum L.) fruits are naturally perishable and have short shelf life. Post-harvest losses can be devastating and can be associated with rapid ripening. The objective of this study was to identify a single nucleotide polymorphism (SNP) marker associated with alc gene conferring long shelf life in tomato. The tomato line CSIR/CRI-AT06 was crossed with Alc-LA3134 to develop an F 2 population. A total of 72 plants were screened for the target loci. Genotypic data of the F 2 population were generated following the scoring system as [T:A] for heterozygous individuals, [A:A] and [T:T] for plants homozygous for the donor and recipient parents, respectively. An assessment of the Sly10-14 SNP marker was completed by comparing SNP score against fruit shelf life of the 72 F 2 entries. The pattern of segregation of SNP marker for the alc gene was tested for goodness of fit to a 1:2:1 ratio using Chi-square test (χ2) test. The results showed that the marker segregated in the expected ratio. The analysis from 72 F 2 population plants indicated that SNP marker (Sly 10-14) had significant association with shelf life at 1% significance level. From the analysis, it can be established that a locus linked to Sly10-14 is significant. The homozygous marker (A:A) has an effect of increasing shelf life by 22 days and explains 87% of the observed variation. This marker (Sly10-14) is linked to a gene that controls the shelf life of tomato fruits. The effect of this gene is additive and it increases the shelf life.
ISSN:1542-7528
1542-7536
DOI:10.1080/15427528.2019.1657216