Chiral Capillary Electrophoretic Determination of 2′,3′-Dideoxy-5-fluoro-3′-thiacytidine in Rat Plasma

• Published in: Journal of liquid chromatography & related technologies 2003-11, Vol.26 (18), p.3037-3044
• Main Authors: Delinsky, David C., Abu-Raddad, Eyas J., Bartlett, Michael G.
• Format: Article
• Language: English
• Subjects: Analysis; Biological and medical sciences; Chiral capillary electrophoresis; Cyclodextrin; Dideoxyfluorothiacytidine; FTC; General pharmacology; Medical sciences; Pharmacology. Drug treatments; RCV
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-120025419

Chiral drugs have the potential for differential pharmacokinetics and metabolism of individual enantiomers. Analytical methods are needed for the separation and quantitation of the enantiomers. Here, we present a method for the separation and quantitation of two enantiomers of 2′,3′-dideoxy-5-fluoro-3′-thiacytidine (FTC) from rat blood plasma using organic protein precipitation with liquid-liquid extraction and capillary electrophoresis (CE). Lamivudine (3TC) was used as an internal standard. The CE system consisted of a 75 µm I.D., 37 cm length fused silica capillary, and a UV detector monitoring a wavelength of 280 nm. The run buffer was aqueous containing 90 mM hydroxypropyl-β-cyclodextrin in 50 mM phosphate at pH 2.5. The system was maintained at 25°C, and the separation voltage was 25 kV with a runtime of 15 min. The method was linear over the range from 0.5 to 100 µg/mL. The method had baseline resolution of the enantiomers and showed high precision and accuracy both within and between runs at three different concentrations, including the lower limit of quantitation (0.5 µg/mL).