Identification and Quantification of Bis-GMA and Teg-DMA Released from Dental Materials by HPLC

• Published in: Journal of liquid chromatography & related technologies 2005-01, Vol.28 (2), p.289-295
• Main Authors: Stefova, Marina, Ivanova, Violeta, Muratovska, Ilijana
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Bis-GMA; Dental materials; HPLC; Identification; Medical sciences; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; TEG-DMA
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-200041340

Most of the composites and sealants used in dentistry are based on bisphenol A diglycidylether methacrylate (Bis-GMA) and triethylenglycol dimethacrylate (Teg-DMA). Reports revealed that in situ polymerization is not complete and that free monomers can be detected by different analytical methods. We studied residual Bis-GMA and Teg-DMA after in vitro polymerization by high performance liquid chromatography (HPLC) after 24 hr, 7, and 14 days. For quantitative analysis, an RP column (250 × 4.6 mm; 5 µm) as stationary phase and isocratic elution with CH 3 CN:H 2 O (60:40) was used. A calibration curve was constructed using standard solutions of Bis-GMA and Teg-DMA in the concentration range from 1 × 10 −3 to 5 × 10 −5  mol/L. The limits of detection (LOD) and quantification (LOQ) for both analytes were determined (for Bis-GMA: 0.0076 and 0.0229 mmol/L; and for Teg-DMA: 0.0123 and 0.0373 mmol/L, respectively). The accuracy of the method was confirmed by standard additions. The HPLC analysis of residual components revealed that Bis-GMA was released from commercial samples in the highest concentration after 14 days. Teg-DMA was detected only in one of all analyzed dental materials.