Lipase from coral associated bacteria: screening, optimizing lipase production, and molecular characterization

• Published in: IOP conference series. Earth and environmental science 2024-07, Vol.1377 (1), p.12087
• Main Authors: Haryati, T, Fatmawati, F, Laksmi, F A, Nuryana, I, Putri, D H, Sa’diah, J N, Khaerunnisa
• Format: Article
• Language: English
• Subjects: Bacteria; Culture media; Industrial applications; Lipase; Olive oil; Optimization; Parameter modification; Rhodamine; rRNA 16S; Selective media; Sequence analysis; Soybean oil; Soybeans; Substrates
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/1377/1/012087

To obtain lipase that can be utilized in industrial applications, it is crucial to explore a new source of lipase. Marine bacteria, especially those associated with coral, are a potential source of lipase. In this study, a total of 50 coral-associated bacteria was screened based on their lypolitic activity on a selective agar plate that contained olive oil and rhodamine B. The highest lypolitic activity was observed on isolate 30.10.11. To obtain a high lipase yield from culture cultivation, we optimized lipase production media using parameters of induction time, substrate oil, nitrogen source, pH, temperature, and % oil induction variation. The optimum condition with lipolytic activity at the level of 0.680 U/mL was obtained by cultivating the coral bacterium on modified Zobel marine media with 0.6% tryptone, 5% soybean oil induction, pH 8, 150 rpm, and 30 °C for 24 hours. Based on the 16S rRNA sequence analysis, the coral bacterium isolate 30.10.11 showed 99.65% similarity to Staphylococcus haemolyticus . Lipase obtained from this isolate is potential to be used in industrial applications, therefore it needs further research on its lipase characterization.