Partial isolation of the starch branching enzymes [SBE] encoding gene from the plantain banana [Musa paradisiaca] genotype Raja

Banana fruit has the potential to be developed as a functional food in terms of its medicinal benefit. One of its features enabling such beneficial uses is its resistant starch characteristic. However, the starch resistance composition of the fruit will decrease during fruit maturation and food proc...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2020-04, Vol.497 (1), p.12019
Main Authors: Syukriani, L, Asben, A, Suliansyah, I, Jamsari, J
Format: Article
Language:English
Subjects:
Amylopectin
Chain branching
Deoxyribonucleic acid
DNA
Food processing
Fruits
functional food
Functional foods & nutraceuticals
Gene expression
Genotypes
Glucan
Metabolism
Nucleotide sequence
Pisang Raja
resistant starch
SBE gene
Starch
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Summary:Banana fruit has the potential to be developed as a functional food in terms of its medicinal benefit. One of its features enabling such beneficial uses is its resistant starch characteristic. However, the starch resistance composition of the fruit will decrease during fruit maturation and food processing. Rationally, blocking of starch resistance decreasing process should maintain the beneficial characteristic of the banana fruit. This could be done by suppressing the expression of the SBE gene so that it can inhibit the formation of branching points in the amylopectin chain metabolism. Based on that consideration, we isolated the SBE gene involved in the formation of amylopectin in genotype Pisang Raja. In the initial step, the MaSBE_5458_F and MaSBE_5458_R primer combination was designed and successfully generated part of the sequence of the MaSBE5 gene. A total DNA sequence of 1,372 bp in length has been isolated, purified and characterized by sequencing technique. Blast analysis showed that the MaSBE_5458 gene sequence has a 97% similarity with 1,4-alpha glucan branching enzyme 1, chloroplastic/amyloplastic DNA sequence isolated from Musa acuminata subsp. malaccensis.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/497/1/012019