Loading…

Enhancement of Indigenous Fungal Cellulase Production by Gamma Rays

The increasing demand for cellulases causes the need for a high cellulase-producing microbe. Mutagenesis is an efficient way to produce a high-titer cellulase-producing strain. Mutagenesis using gamma rays irradiation has the advantage that it can cause a double strand break of DNA. Repair of double...

Full description

Saved in:
Bibliographic Details
Published in:IOP conference series. Materials Science and Engineering 2021-11, Vol.1192 (1), p.12004
Main Authors: Hidayati, F L N, Sardjono, Giyatmi, Cahyanto, M N
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The increasing demand for cellulases causes the need for a high cellulase-producing microbe. Mutagenesis is an efficient way to produce a high-titer cellulase-producing strain. Mutagenesis using gamma rays irradiation has the advantage that it can cause a double strand break of DNA. Repair of double-strand break tends to has an error-prone repair that leads to the alteration of DNA sequence. The aim of this study was to screen high cellulase-producing indigenous fungal mutants produced by mutagenesis. Trichoderma sp. PK1J2 was subjected to gamma irradiation at 300 Gy. The mutants produced were screened using a plate medium containing cellulose as a sole carbon source. After staining with congo red, colonies with wider clear zones were grown in a liquid medium for four days, and the cellulase activities were analyzed. Mutant M8 produces endoglucanase, FPase, and β-glucosidase at 0.46 U/ml, 0.18 U/ml, and 1.10 U/ml, respectively, which were 90%, 50%, and 30% higher than those of the parental strain.
ISSN:1757-8981
1757-899X
DOI:10.1088/1757-899X/1192/1/012004