Mutations in the SLC2A10 gene cause arterial abnormalities in mice

• Published in: Cardiovascular research 2009-02, Vol.81 (2), p.381-388
• Main Authors: Cheng, Chao-Hung, Kikuchi, Tateki, Chen, Yen-Hui, Sabbagha, Nagham George Abd-Al-Ahad, Lee, Yi-Ching, Pan, Huei-Ju, Chang, Chen, Chen, Yuan-Tsong
• Format: Article
• Language: English
• Subjects: Animals; Arterial tortuosity syndrome; Arteries - abnormalities; Arteries - pathology; Biological and medical sciences; Cardiology. Vascular system; Elastic Tissue - cytology; Female; Glucose Transport Proteins, Facilitative - genetics; Glucose transporter 10; Male; Medical sciences; Mice; Mice, Inbred C3H; Mutation; SLC2A10; Transforming Growth Factor beta - physiology
• ISSN: 0008-6363; 1755-3245
• DOI: 10.1093/cvr/cvn319

Aims Glucose transporter 10 (GLUT10), encoded by the SLC2A10 gene, is a member of the class III facilitative glucose transporter family. Mutations in the SLC2A10 gene cause arterial tortuosity syndrome (ATS) in humans. To further study the pathogenesis of the disease, we generated mice carrying GLUT10 mutations. Methods and results Using a gene-driven N-ethyl-N-nitrosourea (ENU)-mutagenesis approach, we generated mice carrying GLUT10 mutations c.383G>A and c.449C>T, which resulted in missense mutations of glycine to glutamic acid (p.G128E) and serine to phenylalanine (p.S150F), respectively. Both mutant strains appeared normal at birth, gained weight appropriately and survived to adulthood (>18 months). Blood and urine glucose were normal. Echocardiogram and electrocardiogram were also normal and brain magnetic resonance angiography revealed normal cerebral arteries without tortuosity, stenosis/dilatation, or aneurysm. Histopathology revealed thickening and irregular vessel wall shape of large and medium size arteries characterized by markedly increased elastic fibres, both in number and size. There was also intima endothelial hypertrophy and deranged elastic fibres that resulted in disruption of internal elastic lamina in the aorta of older mice. Conclusion Abnormal elastogenesis with early elastic fibre proliferation provides a clue to the pathogenesis of arterial tortuosity in human ATS. Availability of this mouse model will allow testing of the relationship between diabetes and its vascular complications, including diabetic retinopathy, nephropathy and peripheral vascular disease.