P157 THE ASSOCIATION STUDY BETWEEN HLA GENOTYPE AND MUCOSAL MICROBIAL COMPOSITION IN PATIENTS WITH INFLAMMATORY BOWEL DISEASES

• Published in: Inflammatory bowel diseases 2019-02, Vol.25 (Supplement_1), p.S72-S72
• Main Authors: Hirano, Atsushi, Shibata, Hiroki, Kakuta, Yoichi, Nagasaki, Masao, Tokunaga, Katsushi, Khor, Seik-Soon, Kawai, Yosuke, Umeno, Junji, Torisu, Takehiro, Kitazono, Takanari, Esaki, Motohiro
• Format: Article
• Language: English
• ISSN: 1078-0998; 1536-4844
• DOI: 10.1093/ibd/izy393.180

Abstract Background Although inflammatory bowel diseases (IBD) is presumed to develop as the result of dysregulated immune response to the intestinal microbiota in genetically susceptible hosts, the association between microbiota and genotypes in IBD patients remains unclear. The human leukocyte antigen (HLA)-Cw*1202-B*5201-DRB1*1502 haplotype has been reported to increase the susceptibility to ulcerative colitis (UC), but reduce the risk of Crohn’s disease (CD). We investigated the association between HLA genotype and mucosal microbial composition to elucidate factors that might affect disease phenotypes among IBD patients. Methods Mucosal bioptic sampling was performed from the rectum under colonoscopy for the analysis of mucosal microbial composition among 54 IBD patients (27 patients with CD and 27 patients with UC). The mucosal microbial community structure was investigated using 16S rRNA gene sequences, and the structures were analyzed using Qiime and LEfSe software. All patients were genotyped using the Affymetrix Japonica Array, and their HLA genotypes were determined by imputation based on the Japanese-specific references. Results Mucosal microbial structure was significantly different between CD patients and UC patients. Six CD patients and 15 UC patients had the HLA-Cw*1202-B*5201-DRB1*1502 allele. Among patients with CD, microbes of the genera Ruminococcus, Leptolyngbya, Clostridium, Comamonas and Aggregatibacter were more prevalent in HLA-Cw*1202-B*5201-DRB1*1502 carriers when compared to non-carriers. In contrast, decreased abundance of the genus Acidaminococcus and increased abundance of genera Veillonella, SMB53, Lachnospira and Haemophilus was evident in HLA-Cw*1202-B*5201-DRB1*1502 carriers when compared to non-carriers among patients with UC. Conclusion While mucosal microbiota composition was different between CD and UC, HLA-Cw*1202-B*5201-DRB1*1502 allele might affect mucosal microbiota composition in both CD and UC.