Fracture characteristics of human cortical bone influenced by the duration of in vitro glycation

Advanced glycation end products (AGEs) accumulate in various tissues, including bone, due to aging and conditions like diabetes mellitus. To investigate the effects of AGEs on bone material quality and biomechanical properties, an in vitro study utilized human tibia, sectioned into 90 beams, and ran...

Full description

Saved in:
Bibliographic Details
Published in:JBMR plus 2024-11
Main Authors: Lin, Mei-Chun, Sihota, Praveer, Kolibová, Sofie Dragoun, Fiedler, Imke A K, Krug, Johannes, Wölfel, Eva M, Moritz, Manuela, Riedner, Maria, Ondruschka, Benjamin, Citak, Mustafa, Klebig, Felix, von Brackel, Felix N, Qwamizadeh, Mahan, Jähn-Rickert, Katharina, Busse, Björn
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Advanced glycation end products (AGEs) accumulate in various tissues, including bone, due to aging and conditions like diabetes mellitus. To investigate the effects of AGEs on bone material quality and biomechanical properties, an in vitro study utilized human tibia, sectioned into 90 beams, and randomly assigned to three mechanical test groups. Each test group included ribose (c=0.6 M) treatment at 7-, 14-, and 21-day, alongside control groups (n=5 per group). Fluorescent AGE (fAGE) and carboxymethyl-lysine (CML) levels were assessed through fluorometric analysis and mass spectrometry, while bone matrix composition was characterized using Fourier-transform infrared and Raman spectroscopy. Mechanical properties were determined through nanoindentation and three-point bending tests on non-notched and notched specimens. The results showed significant increases in fAGEs levels at 7-, 14-, and 21-day compared to controls (119%, 311%, 404%; p=0.008, p
ISSN:2473-4039
2473-4039
DOI:10.1093/jbmrpl/ziae151