Fluorosensor proteins to detect specific DNA sequences in living bacteria

While there are many hybridization-based DNA sensors, few of them can detect native double-stranded DNA, which is most commonly found in physiological conditions. Here we made novel fluorosensor proteins comprised of a pair of two zinc fingers tethered with an N-terminal dimerization motif and a C-t...

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 2007-11, Vol.51 (1), p.121-122
Main Authors: Waki, Shoko, Yoshitake, Kazutoshi, Iwasaki, Ryohei, Ueda, Hiroshi
Format: Article
Language:English
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Summary:While there are many hybridization-based DNA sensors, few of them can detect native double-stranded DNA, which is most commonly found in physiological conditions. Here we made novel fluorosensor proteins comprised of a pair of two zinc fingers tethered with an N-terminal dimerization motif and a C-terminal yellow fluorescent protein fragment (split eYFP) to detect specific DNA sequence in a living bacteria. When E. coli Top10 cells harboring the plasmid encoding the fusion proteins and a test plasmid encoding target DNA sequence were induced for the protein expression, significant increase in fluorescence was observed, compared with the strain harboring a test plasmid without target DNA sequence.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/nrm061