New triarylamine-based far-red DNA stainers with high two-photon absorption properties

Abstract A series of red emitting vinyl-triphenyamines (TP) have been synthesized and evaluated for their two photon absorption (2PA) properties. These compounds are virtually non fluorescent in the free state but exhibit a bright red fluorescence upon binding to doublestranded DNA, both in one- and...

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 2008, Vol.52 (1), p.155-156
Main Authors: Bordeau, G., Faurel, E., Allain, C., Lartia, R., Schmidt, F., Fiorini-Debuisschert, C., Charra, F., Metge, G., Tauc, P., Teulade-Fichou, M.-P.
Format: Article
Language:English
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Summary:Abstract A series of red emitting vinyl-triphenyamines (TP) have been synthesized and evaluated for their two photon absorption (2PA) properties. These compounds are virtually non fluorescent in the free state but exhibit a bright red fluorescence upon binding to doublestranded DNA, both in one- and two-photon absorption. This feature allows one- and two-photon confocal imaging in cells of nuclear DNA with an excellent contrast. Derivatizable analogues for covalent bioconjugation to oligonucleotides are described and variation on the structure is discussed.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/nrn079