Recognition and reaction mechanisms of the (6-4) photolyase as determined by using a (6-4) photoproduct analog

Abstract The (6-4) photoproduct, which is one of the major UV-induced DNA lesions formed at bipyrimidine sites, causes carcinogenesis at high frequency. The (6-4) photolyases restore the (6-4) photoproducts to their intact bases in a light-dependent manner, but its overall repair mechanism remains o...

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Bibliographic Details
Published in:Nucleic Acids Symposium Series 2009, Vol.53 (1), p.221-222
Main Authors: Yamamoto, Junpei, Hitomi, Kenichi, Hayashi, Ryosuke, Getzoff, Elizabeth D., Iwai, Shigenori
Format: Article
Language:English
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Summary:Abstract The (6-4) photoproduct, which is one of the major UV-induced DNA lesions formed at bipyrimidine sites, causes carcinogenesis at high frequency. The (6-4) photolyases restore the (6-4) photoproducts to their intact bases in a light-dependent manner, but its overall repair mechanism remains obscure. To investigate the light-dependent conversion of the (6-4) photoproduct, we prepared a (6-4) photoproduct analog with modification at 3′ pyrimidone ring, in which the carbonyl group was replaced with an imine to apply to the (6-4) photolyase assay. The (6-4) photolyase shows affinity to an oligonucleotide carrying this imine analog of the (6-4) photoproduct, though the imine analog is not repaired by the (6-4) photolyase.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/nrp111