A new method for determining the availability of choline in soybean meal

• Published in: Poultry science 1997-09, Vol.76 (9), p.1292-1297
• Main Authors: Menten, J.F.M, Pesti, G.M, Bakalli, R.I
• Format: Article
• Language: English
• Subjects: Animals; BESOIN NUTRITIONNEL; BIOAVAILABILITY; BIODISPONIBILIDAD; BIODISPONIBILITE; Biological Availability; BROILER CHICKENS; Chickens - growth & development; Chickens - metabolism; Chickens - physiology; CHICKS; CHOLINE; Choline - administration & dosage; Choline - analysis; Choline - pharmacokinetics; CISTINA; COLINA; Cysteine - administration & dosage; Cysteine - analysis; Cysteine - pharmacokinetics; CYSTINE; Dietary Proteins - administration & dosage; Dietary Proteins - analysis; Dietary Proteins - pharmacokinetics; Dietary Supplements; DOSAGE; DOSIFICACION; Eating - physiology; FEED INTAKE; GAIN DE POIDS; GANANCIA DE PESO; Glucose - administration & dosage; Glucose - analysis; Glucose - pharmacokinetics; Glycine max - chemistry; Glycine max - metabolism; GROWTH RATE; INDICE DE CRECIMIENTO; INGESTION DE NUTRIENTES; INGESTION DE PIENSOS; INGESTION DE SUBSTANCES NUTRITIVES; LIVEWEIGHT GAIN; Male; METHIONINE; Methionine - administration & dosage; Methionine - analysis; Methionine - pharmacokinetics; METIONINA; NECESIDADES DE NUTRIENTES; NUTRIENT INTAKE; NUTRITIONAL REQUIREMENTS; POLLITO; POLLO DE ENGORDE; POULET DE CHAIR; POUSSIN; PRISE ALIMENTAIRE (ANIMAUX); Random Allocation; Regression Analysis; Reproducibility of Results; SOYBEAN MEAL; SOYBEAN OILMEAL; TAUX DE CROISSANCE; TORTAS DE SOJA; TOURTEAU DE SOJA; WEIGHT GAIN; Weight Gain - physiology
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1093/ps/76.9.1292

Studies were conducted to evaluate the availability of choline in soybean meal, using a new approach: The basal diet contained soybean meal with a lowered choline content (partially extracted with methanol). Choline was added to the basal diet (1) by substituting intact soybean meal for the methanol washed soybean meal; or (2) from crystalline choline Cl. Four experiments were conducted with day-old male broilers housed in battery brooders and fed the experimental diets from 4 to 18 d posthatching. The basal diet contained corn, soybean meal, and isolated soy protein in Experiments 1 to 3, and also glucose in Experiment 4. In Experiment 1, chicks fed the basal diet (1,140 mg/kg choline, 0.61% sulfur amino adds) had a growth response to 1,000 mg/kg choline or 0.10% methionine (P 0.05), but not to 0.10% cysteine (P 0.05) supplementation. Supplementation of choline (0, 50, 100, 200, 400, 800, and 1,200 mg/kg) to the basal diet (1,230 mg/kg choline) in Experiment 2 resulted in a linear increase in growth up to 122 +/- 22 mg/kg supplemental choline (1,352 +/- 22 mg/kg total choline), reaching a plateau after that. In the slope ratio assays to determine choline availability, the basal diets in Experiments 3 (1,098 mg/kg choline) and 4 (920 mg/kg choline) were supplemented with either 50 or 100 mg/kg choline (from choline Cl) or had approximately 50 or 100 mg/kg choline added with intact soybean meal (at the expense of washed soybean meal). There were significant linear responses of weight gain vs choline intake from either source (P 0.001). The availability of choline in soybean meal was calculated to be 97 and 105% in Experiments 3 and 4, respectively. These results indicate that choline availability in soybean meal is close to 100%