Post Infarction High Saturated Fat Feeding Does Not Exacerbate LV Dysfunction but Enhances Mitochondrial Function

• Published in: The FASEB journal 2006-03, Vol.20 (4), p.A316-A316
• Main Authors: Rennison, Julie Helene, McElfresh, Tracy A, Okere, Isidore C, Vazquez, Edwin J, Patel, Hiral V, Foster, Amy B, Patel, Kalpana K, Chen, Qun, Hoit, Brian D, Hoppel, Charles L, Chandler, Margaret P
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.20.4.A316

Accumulation of lipid and ceramide in the heart is associated with contractile dysfunction, and has been proposed to be a causative factor in mitochondrial dysfunction and decreased electron transport chain (ETC) activity. We hypothesized that a high saturated fat diet (SAT) would exacerbate left ventricular (LV) dysfunction and decrease mitochondrial respiration and ETC complex activity. Heart failure was induced by coronary artery ligation. Rats were fed normal chow (10% kcal from fat, HF, n=8) or SAT (60% kcal from saturated fat, HF FAT, n=7) for 8 weeks. Sham rats (SHAM, n=8) were fed normal chow. LV function was assessed by echocardiography. Interfibrillar (IFM) and subsarcolemmal (SSM) mitochondria were isolated from the LV and ETC complex activities and oxidative phosphorylation were assessed. Peak LV +/−dP/dt were decreased with HF (+4466 ± 487 and −3959 ± 396 mmHg/min) compared to SHAM (+6134 ± 526 and −5569 ± 401 mmHg/min) (p