New Sugar Inhibitors of Immobilized Concanavalin A Binding

• Published in: The FASEB journal 2007-04, Vol.21 (5), p.A260-A261
• Main Authors: Zem, Gregory C, Goldstein, Laurie, Ortega, Joao D., Amireh‐Chamgi, Liona, Lim, Joanne H., Khanjian, Harout, McNicholas, Erin, Cheung, Candice, Arman, Odette, Budiyono, Herry, Ngotngamwong, Peter, Hudgens, Grace, Davoodpour, Monica, Farahani, Shabnam, Sardroud, Bernadet Aziz, Zhamkochyan, Suzie, Zallaghi, Forough, Luna, Lourdes, Gonzalez, Karime, Julfayan, Ani, Oppenheimer, Steven B.
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.21.5.A260-d

We reported an inexpensive alternative to microarrays in the development of carbohydrate drugs and diagnostic tests using lectin derivatized microbeads. In addition, we developed arguably the most extensive listing of 30 saccharide inhibitors of Concanavalin A (Con A) binding to mannose‐rich yeast in order of decreasing inhihitor effectiveness (Zem et al., Acta Histochemica 108: –317, 2006). In this study, we examine Con A binding to Saccharomyces cerivisiae in the presence and absence of sugars that we have not tested previously. We incubated three times washed yeast (1 mg/ml) with three times washed agarose beads derivatized with Con A (2 mg/ml), in 50 microliter droplets on microscope slides with or without lactulose, palatinose, D(+)turanose, or sucrose, as well as two previously studied sugars, D‐galactose and maltose, all at 0.05 M. A total of 5,474 trials were performed in which we scored the samples incubated with each sugar as inhibiting yeast binding to Con A beads, compared with binding in the absence of sugar, promoting binding or no effect at all. Palatinose, turanose and maltose were better inhibitors of binding than lactulose, galactose and sucrose. This study and our previous work (Zem, et al., cited above) provide an extensive listing of sugar inhibitors of Con A binding, some of which are more effective inhibitors that those that are used in most experiments (alpha‐methyl‐mannose and alpha‐methyl‐glucose) (Supported by NIH NIGMS SCORE, RISE, MARC and the Joseph Drown Foundation).