Impact of High Fat Diet and Injury on Carotid Artery Reactivity

• Published in: The FASEB journal 2007, Vol.21 (6), p.A868-A868
• Main Authors: Gandley, Robin E., Raman, Kathleen G., Rohland, Jennifer R., Zenati, Mazen S., Tzeng, Edith
• Format: Article
• Language: English
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.21.6.A868-c

OBJECTIVE: To determine the impact of a high fat diet on carotid artery injury response in an ApoE mouse model. METHODS: C57BL/6J (C57) and ApoE−/− mice were fed a Western‐type (WTD) or chow diet (CD) for 4 wks, and common carotid arteries (CCA) were isolated and injured with a .014 inch wire. Animals recovered and remained on the diets for 2 or 4 weeks. Injured (INJ) and intact (INT) CCA were removed and mounted in a pressurized dual arteriograph. Myogenic reactivity (pressure stimulated response), endothelial (methacholine, ME) and non‐endothelial (sodium nitroprusside, SNP) mediated relaxation were assessed. Data were analyzed by ANOVA with 4‐6 mice/group. RESULTS: WTD reduced the maximal relaxation to ME in INT arteries at 4 weeks post‐injury (C57/CD=75.5% relaxation vs. C57/WTD= 50.5% p=0.004, ApoE/CD= 55.5% vs. ApoE/WTD= 38.8% p=0.02). Maximal ME relaxation was impaired in INJ CCA from ApoE mice on WTD at 2 weeks 6.8% vs. INT CCA 70% (p