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Effects of Resveratrol in CXCR4 Expression and Apoptotic Markers in Normal Cell Lines Derived from a Breast Cancer Patient

Abstract only The cellular and molecular environment of normal cells from breast cancer patients are particulary understood. Chemokines and their receptors have roles in cellular proliferation, metastasis, and angiogenesis. Specifically, CXCR4, a G‐protein coupled receptor is expressed in high level...

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Bibliographic Details
Published in:The FASEB journal 2016-04, Vol.30 (S1)
Main Authors: Arroyo, Gerardo Antonio, Figueroa, Maria, Muñoz‐Forti, Kevin, Maldonado, Alexandra, Torres, Bryan, Bernard, Faviola, Almodovar, Sharilyn, Suarez, Edu, Ruiz, Abigail
Format: Article
Language:English
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Summary:Abstract only The cellular and molecular environment of normal cells from breast cancer patients are particulary understood. Chemokines and their receptors have roles in cellular proliferation, metastasis, and angiogenesis. Specifically, CXCR4, a G‐protein coupled receptor is expressed in high levels in breast neoplastic tissues and in low levels in normal breast tissue. CXCR4 expression may induce proliferation, migration, invasion and angiogenesis. Recent studies have identify resveratrol (a natural polyphenol) to play a role on inflammation, apoptosis, and neovascularization. Due to limited scientific literature on the effects of resveratrol on CXCR4, the biological process in which both coincide, (inflammation, apoptosis, and neovascularization), and the potential use of resveratrol, as a therapeutic agent in cancer, we studied the effect of this polyphenol on CXCR4. In addition, we explored the effects on the apoptosis mediator Bax. We hypothesized that resveratrol increases the expression of CXCR4 in normal cells from a breast cancer patient. We determined the effects of resveratrol at concentrations of 25, 50, 100, 200 μM after 24 hours of treatment in the protein levels of CXCR4 and Bax‐2 by western blot using CCD1097 a fibroblastic cell line, derived from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma. Our preliminary results indicate that higher doses increased expression of CXCR4 in a dose‐dependent compared to control vehicle treated cells. Similarly, Bax expression increased in all treated cells but in a non‐dependent dose manner. These results may suggest that resveratrol have an effect on CXCR4 protein levels, leading to an increase in proliferation in normal cells from cancer patients. As reported, at lower doses resveratrol may have antiapoptotic effects and at higher doses may induce apoptosis. Resveratrol may prompt for clearance of cancer cells using different pathways but additional experiments will be necessary to identify the exact mechanism of action. Additional experiments are underway to identify the proliferative effects of resveratrol including apoptosis (caspases) and inflammation (TNF‐α and SOD‐2). Support or Funding Information This work was supported by UPR PRISE Program NIH Grant #R25GM096955.
ISSN:0892-6638
1530-6860
DOI:10.1096/fasebj.30.1_supplement.lb47