Ecto-5'-nucleotidase (CD73) -mediated extracellular adenosine production plays a critical role in hepatic fibrosis

• Published in: The FASEB journal 2008-07, Vol.22 (7), p.2263-2272
• Main Authors: Peng, Zhongsheng, Fernandez, Patricia, Wilder, Tuere, Yee, Herman, Chiriboga, Luis, Chan, Edwin S.L, Cronstein, Bruce N
• Format: Article
• Language: English
• Subjects: 5'-Nucleotidase - deficiency; 5'-Nucleotidase - genetics; 5'-Nucleotidase - physiology; Adenosine - biosynthesis; Animals; carbon tetrachloride; Carbon Tetrachloride - toxicity; Disease Models, Animal; Extracellular Space - physiology; Liver Cirrhosis - enzymology; Liver Cirrhosis - genetics; Liver Cirrhosis - physiopathology; Matrix Metalloproteinases - genetics; Mice; Mice, Inbred C57BL; Mice, Knockout; Receptors, Purinergic P1 - genetics; Reference Values; stellate cell; thioacetamide
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.07-100685

Adenosine is a potent endogenous regulator of tissue repair that is released from injured cells and tissues. Hepatic fibrosis results from chronic hepatic injury, and we have previously reported that endogenously generated adenosine, acting at A₂A receptors, plays a role in toxin-induced hepatic fibrosis. Adenosine may form intracellularly and then be transported to the extracellular space or it may form extracellularly from adenine nucleotides released from injured cells. Because ecto-5'-nucleotidase (CD73) catalyzes the terminal step in extracellular adenosine formation from AMP, we determined whether CD73 plays a role in the development of hepatic fibrosis. Mice were treated overnight with PBS, CCl₄, ethanol, or thioacetamide (TAA); their livers were harvested, and slices were incubated in medium for 20 h before adenosine concentration in the supernatant was measured by HPLC. Hepatic fibrosis was induced by CCl₄ or TAA treatment in CD73 knockout (CD73KO and C57BL/6 background) and C57BL/6 control mice [wild-type (WT)] mice and quantified by digital analysis of picrosirius red stained slides and hydroxyproline content. mRNA expression was quantified by real-time polymerase chain reaction, and protein was quantified by Western blot or enzyme-linked immunosorbent assay. Livers from WT mice treated with CCl₄, ethanol, and TAA released 2- to 3-fold higher levels of adenosine than livers from comparably treated CD73KO mice. CD73KO mice were protected from fibrosis with significantly less collagen content in the livers of CD73KO than WT mice after treatment with either CCl₄ or TAA. There were far fewer α-smooth muscle actin positive hepatic stellate cells in CCl₄-treated KO mice than that in WT mice. After CCl₄ treatment, the mRNA level of A₁, A₂A, A₂B, and A₃ adenosine receptors, tumor necrosis factor-α, interleukin (IL) -1β, IL-13rα1, matrix metalloproteinase (MMP)-2, MMP-14, tissue inhibitor of metalloproteinase (TIMP) -1, and TIMP-2, and IL-13 level increased markedly in both CD73KO and WT mice, but Col1α1, Col3α1, and transforming growth factor-β1 mRNA increased much more in WT mice than that in KO mice. Moreover, IL-13rα2, MMP-13 mRNA, and MMP-13 protein were higher in KO mice than that in WT mice. These results indicate that adenosine, formed extracellularly from adenine nucleotides, plays a major role in the pathogenesis of hepatic fibrosis and that inhibition of adenosine production or blockade of adenosine receptors may help prevent hepatic fibrosis.