PS1312 QPCR, MFC AND DDPCR: COMPARISON ON MRD SAMPLES FROM THREE PROSPECTIVE TRIALS OF THE EUROPEAN MCL NETWORK

Background: Minimal residual disease (MRD) detection in mantle cell lymphoma (MCL) provides valuable prognostic information, leading to the design of MRD‐based therapeutic strategies in prospective clinical trials. Quantitative polymerase chain reaction (qPCR) of IGH or BCL1‐IgH rearrangements is th...

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Published in:HemaSphere 2019-06, Vol.3 (S1), p.599-n/a
Main Authors: Drandi, D., Alcantara, M., Barbero, D., Benmaad, I., Lhermitte, L., Ferrante, M., Zaccaria, G.M., Mantoan, B., Genuardi, E., Ruggeri, M., Omedè, P., Villarese, P., Cheminant, M., Cortelazzo, S., Pott, C., Dreyling, M., Hermine, O., Delfau‐Larue, M.‐H., Ladetto, M., Ferrero, S., Macintyre, E.
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Language:English
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Summary:Background: Minimal residual disease (MRD) detection in mantle cell lymphoma (MCL) provides valuable prognostic information, leading to the design of MRD‐based therapeutic strategies in prospective clinical trials. Quantitative polymerase chain reaction (qPCR) of IGH or BCL1‐IgH rearrangements is the gold standard for MRD monitoring in MCL. Its major limitation is its relative quantification and the high proportion of follow‐up (FU) samples with a low tumor burden that fall below the quantitative range (BQR) of the standard curve. Multiparameter flow cytometry (MFC) is a rapid absolute quantification alternative to qPCR which showed promise on retrospective testing. (Cheminant M. et al.) Aims: We here compare qPCR, prospective MFC and ddPCR in MCL samples from three laboratories of the Euro‐MRD consortium. The main aim was to compare prospective MFC and ddPCR, particularly in FU samples with a low tumor burden, defined as BQR by qPCR Methods: Samples were collected (with informed consent) in three prospective EU‐MCL network clinical trials. qPCR and ddPCR were performed in Paris‐Necker, Creteil and Torino according to Euro‐MRD guidelines, as described (van der Velden VH. et al, Drandi D. et al). Based on Poisson assumption, we stratified results with alternatively positive or negative replicates into three subgroups‐ for qPCR: 3/3 BQR vs. 2/3 vs. 1/3 positive replicates; for ddPCR: 2/3 or 1/3 replicates with > = 3 positive events vs. 2/3 replicates with
ISSN:2572-9241
2572-9241
DOI:10.1097/01.HS9.0000563528.69483.e1