SCF ubiquitin protein ligases and phosphorylation–dependent proteolysis

Many key activators and inhibitors of cell division are targeted for degradation by a recently described family of E3 ubiquitin protein ligases termed Skp1-Cdc53-F-box protein (SCF) complexes. SCF complexes physically link substrate proteins to the E2 ubiquitin-conjugating enzyme Cdc34, which cataly...

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Bibliographic Details
Published in:Philosophical transactions of the Royal Society of London. Series B. Biological sciences 1999-09, Vol.354 (1389), p.1533-1550
Main Authors: Willems, A. R., Goh, T., Taylor, L., Chernushevich, I., Shevchenko, A., Tyers, M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antibodies
Carrier Proteins
Cdc34
Cell Cycle
Cell Cycle Proteins - metabolism
Cullin Proteins
Cyclin-Dependent Kinase
Cyclins
Cyclins - metabolism
E3 Ubiquitin Protein Ligase
Enzymes
F box proteins
Fungal Proteins - metabolism
Gene expression regulation
Molecular Sequence Data
Peptide Synthases - metabolism
Phosphorylation
Proteins
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
SKP Cullin F-Box Protein Ligases
Skpl-Cdc53-F-Box Protein
Substrate Specificity
Ubiquitin-Protein Ligases
Ubiquitins
Yeasts
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Summary:Many key activators and inhibitors of cell division are targeted for degradation by a recently described family of E3 ubiquitin protein ligases termed Skp1-Cdc53-F-box protein (SCF) complexes. SCF complexes physically link substrate proteins to the E2 ubiquitin-conjugating enzyme Cdc34, which catalyses substrate ubiquitination, leading to subsequent degradation by the 26S proteasome. SCF complexes contain a variable subunit called an F-box protein that confers substrate specificity on an invariant core complex composed of the subunits Cdc34, Skp1 and Cdc53. Here, we review the substrates and pathways regulated by the yeast F-box proteins Cdc4, Grr1 and Met30. The concepts of SCF ubiquitin ligase function are illustrated by analysis of the degradation pathway for the G1 cyclin Cln2. Through mass spectrometric analysis of Cdc53 associated proteins, we have identified three novel F-box proteins that appear to participate in SCF-like complexes. As many F-box proteins can be found in sequence databases, it appears that a host of cellular pathways will be regulated by SCF-dependent proteolysis.
ISSN:0962-8436
1471-2970
DOI:10.1098/rstb.1999.0497