Modern chromatographic method for estimating Loratadine and affections on healthcare

• Published in: International journal of human rights in healthcare 2024-08, Vol.17 (4), p.507-520
• Main Authors: Al-Salman, H.N.K., Qasim, Qutaiba A., Fayadh, Rajaa Hussein, Hussein, Hussein H.
• Format: Article
• Language: English
• Subjects: Chromatography; Flow rates; Harmonization; Health care; Histamine; Liquid chromatography; Measurement; Potassium; Temperature
• ISSN: 2056-4902; 2056-4902; 2056-4910
• DOI: 10.1108/IJHRH-08-2021-0154

Purpose The purpose of this study is to establish Loratadine [LRD] quantification in purified and capsule formulations using a precise and specific Reversal Phase with a very high-performance liquid Chromatographic [RP-HPLC] technique. The approach was evaluated in agreement with the principles of the International Conference on Harmonization [ICH]. Arcus EP-C18 Ion Pac column, 5 m, 4.6 mm, 250 mm, mobile phase Methanol: Acetonitrile (60:40) v/v. Dibasic potassium phosphate buffer, pH 7.2, flow rate 1.0 ml/min. Design/methodology/approach The HPLC system used a 340 nm UV detector for testing. A 10-min run time was used for the analysis. At concentrations ranging from 2 to 10 g/ml, the technique was linear (R2 = 0.9998), exact (intra-day and inter-day relative standard deviation [RSD] values 1.0%), accurate (range recovery = 96%–102%), exclusive and strong. Findings The detecting and quantitation limits were 0.92 g/ml and 2.15 g/ml, respectively. Originality/value The findings demonstrated that the proposed method could accurately determine LRD in bulk and pill dose formats quickly and accurately.