Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study

The newly developed Asp ID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid ( BALF ) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clini...

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Bibliographic Details
Published in:Mycoses 2018-06, Vol.61 (6), p.355-359
Main Authors: Prattes, Juergen, Hoenigl, Martin, Zinke, Stefanie E.‐M., Heldt, Sven, Eigl, Susanne, Johnson, Gemma L., Bustin, Stephen, Stelzl, Evelyn, Kessler, Harald H.
Format: Article
Language:English
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Summary:The newly developed Asp ID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid ( BALF ) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis ( IPA ) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the Asp ID assay were 94.1% (95% CI 73.3‐99.9), 76.5% (95% CI 50.1‐93.2), 4 (95% CI 1.7‐9.5) and 0.1 (95% CI 0.01‐0.5) respectively.
ISSN:0933-7407
1439-0507
DOI:10.1111/myc.12757