A reactive oxygen species Ca 2+ signalling pathway identified from a chemical screen for modifiers of sugar-activated circadian gene expression

Sugars are essential metabolites for energy and anabolism that can also act as signals to regulate plant physiology and development. Experimental tools to disrupt major sugar signalling pathways are limited. We performed a chemical screen for modifiers of activation of circadian gene expression by s...

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Published in:The New phytologist 2022-11, Vol.236 (3), p.1027-1041
Main Authors: Li, Xiang, Deng, Dongjing, Cataltepe, Gizem, Román, Ángela, Buckley, Christopher R, Cassano Monte-Bello, Carolina, Skirycz, Aleksandra, Caldana, Camila, Haydon, Michael J
Format: Article
Language:English
Subjects:
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Calmodulin - metabolism
Carbohydrates - pharmacology
Circadian Rhythm - physiology
Gene Expression
Gene Expression Regulation, Plant
Pentamidine - metabolism
Pentamidine - pharmacology
Reactive Oxygen Species - metabolism
Sucrose - metabolism
Sugars - metabolism
Superoxides - metabolism
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Summary:Sugars are essential metabolites for energy and anabolism that can also act as signals to regulate plant physiology and development. Experimental tools to disrupt major sugar signalling pathways are limited. We performed a chemical screen for modifiers of activation of circadian gene expression by sugars to discover pharmacological tools to investigate and manipulate plant sugar signalling. Using a library of commercially available bioactive compounds, we identified 75 confident hits that modified the response of a circadian luciferase reporter to sucrose in dark-adapted Arabidopsis thaliana seedlings. We validated the transcriptional effect on a subset of the hits and measured their effects on a range of sugar-dependent phenotypes for 13 of these chemicals. Chemicals were identified that appear to influence known and unknown sugar signalling pathways. Pentamidine isethionate was identified as a modifier of a sugar-activated Ca signal that acts as a calmodulin inhibitor downstream of superoxide in a metabolic signalling pathway affecting circadian rhythms, primary metabolism and plant growth. Our data provide a resource of new experimental tools to manipulate plant sugar signalling and identify novel components of these pathways.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.18380