Can hydraulic design explain patterns of leaf water isotopic enrichment in C 3 plants?

• Published in: Plant, cell and environment cell and environment, 2021-02, Vol.44 (2), p.432-444
• Main Authors: Barbour, Margaret M, Loucos, Karen E, Lockhart, Erin L, Shrestha, Arjina, McCallum, Daniel, Simonin, Kevin A, Song, Xin, Griffani, Danielle S, Farquhar, Graham D
• Format: Article
• Language: English
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.13943

H O enrichment develops when leaves transpire, but an accurate generalized mechanistic model has proven elusive. We hypothesized that leaf hydraulic architecture may affect the degree to which gradients in H O develop within leaves, influencing bulk leaf stable oxygen isotope enrichment (Δ ) and the degree to which the Péclet effect is relevant in leaves. Leaf hydraulic design predicted the relevance of a Péclet effect to Δ in 19 of the 21 species tested. Leaves with well-developed hydraulic connections between the vascular tissue and the epidermal cells through bundle sheath extensions and clear distinctions between palisade and spongy mesophyll layers (while the mesophyll is hydraulically disconnected) may have velocities of the transpiration stream such that gradients in H O develop and are expressed in the mesophyll. In contrast, in leaves where the vascular tissue is hydraulically disconnected from the epidermal layers, or where all mesophyll cells are well connected to the transpiration stream, velocities within the liquid transport pathways may be low enough that gradients in H O are very small. Prior knowledge of leaf hydraulic design allows informed selection of the appropriate Δ modelling framework.