Sensitive HLA antibody testing and the risk of antibody‐mediated rejection and graft failure

Solid phase detection and identification of HLA antibodies in kidney transplantation currently relies on single antigen bead (Luminex®) assays, which is more sensitive than the previously used enzyme‐linked immunosorbent assays (ELISA). To evaluate the impact of more sensitive HLA testing on antibod...

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Bibliographic Details
Published in:HLA : immune response genetics 2024-06, Vol.103 (6), p.n/a
Main Authors: Debyser, Tim, Callemeyn, Jasper, Coemans, Maarten, Kerkhofs, Johan, Koshy, Priyanka, Kuypers, Dirk, Senev, Aleksandar, Tambur, Anat R., Van Loon, Elisabet, Wellekens, Karolien, Naesens, Maarten, Emonds, Marie‐Paule
Format: Article
Language:English
Subjects:
graft rejection
histocompatibility testing
HLA antigens
kidney transplantation
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Summary:Solid phase detection and identification of HLA antibodies in kidney transplantation currently relies on single antigen bead (Luminex®) assays, which is more sensitive than the previously used enzyme‐linked immunosorbent assays (ELISA). To evaluate the impact of more sensitive HLA testing on antibody‐mediated rejection (AMR) occurrence and allograft survival, we analysed 1818 renal allograft recipients transplanted between March 2004 and May 2021. In 2008, solid phase testing switched from ELISA to Luminex. We included 393 (21.6%) transplantations before and 1425 (78.4%) transplantations after transition from ELISA‐ to Luminex‐based testing. For this study, bio‐banked ELISA era samples were tested retrospectively with Luminex. Significantly less pretransplant DSA were found in patients transplanted with pre‐existing HLA antibodies in the Luminex (109/387) versus the ELISA period (43/90) (28% vs. 48%, p 
ISSN:2059-2302
2059-2310
DOI:10.1111/tan.15586