Biological and Phylogenetic Characterization of Pigeon Paramyxovirus Serotype 1 Circulating in Wild North American Pigeons and Doves

As part of West Nile virus surveillance programs in Rhode Island and eastern Texas between 2000 and 2007, brain tissue was collected from 5,608 dead birds representing 21 avian orders found in public places or reported by homeowners. Fifteen Newcastle disease virus isolates were recovered only from...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2008-10, Vol.46 (10), p.3303-3310
Main Authors: Kim, L. Mia, King, Daniel J, Guzman, Hilda, Tesh, Robert B, da Rosa, Amelia P.A. Travassos, Bueno, Rudy Jr, Dennett, James A, Afonso, Claudio L
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Biological and medical sciences
bird diseases
Brain - virology
Columbidae - virology
doves
Fundamental and applied biological sciences. Psychology
Genotype
Hemagglutination Inhibition Tests
Microbiology
Miscellaneous
Molecular Sequence Data
Neutralization Tests
Newcastle Disease - virology
Newcastle disease virus
Newcastle disease virus - classification
Newcastle disease virus - genetics
Newcastle disease virus - isolation & purification
Newcastle disease virus - pathogenicity
North America
Paramyxoviridae
Phylogeny
pigeons
RNA, Viral - genetics
Sequence Analysis, DNA
Sequence Homology
Serotyping
viral diseases of animals and humans
Viral Plaque Assay
Virology
Virulence
wild birds
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Summary:As part of West Nile virus surveillance programs in Rhode Island and eastern Texas between 2000 and 2007, brain tissue was collected from 5,608 dead birds representing 21 avian orders found in public places or reported by homeowners. Fifteen Newcastle disease virus isolates were recovered only from birds of the order Columbiformes and were positively identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gene and more specifically as pigeon paramyxovirus serotype 1 (PPMV-1) by hemagglutinin inhibition with monoclonal antibodies. Based upon partial genomic sequencing and phylogenetic analysis, the newly isolated viruses represent a distinct sublineage within class II genotype VIb. All of the viruses (15/15) were classified as virulent based upon their fusion cleavage site motif (¹¹²RRKKRF¹¹⁷) and intracerebral pathogenicity indices of >0.7 (ranging from 0.98 to1.35); however, these viruses escaped detection by the fusion gene-based real-time PCR test for virulence. Modifications introduced to the probe site of the fusion gene-based assay allowed rapid virulence detection within this distinct sublineage.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00644-08