Glycolysis Controls Plasma Membrane Glucose Sensors To Promote Glucose Signaling in Yeasts

Sensing of extracellular glucose is necessary for cells to adapt to glucose variation in their environment. In the respiratory yeast Kluyveromyces lactis , extracellular glucose controls the expression of major glucose permease gene RAG1 through a cascade similar to the Saccharomyces cerevisiae Snf3...

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Bibliographic Details
Published in:Molecular and cellular biology 2015-02, Vol.35 (4), p.747-757
Main Authors: Cairey-Remonnay, Amélie, Deffaud, Julien, Wésolowski-Louvel, Micheline, Lemaire, Marc, Soulard, Alexandre
Format: Article
Language:English
Subjects:
Biological Transport
Cell Membrane
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Feedback, Physiological
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Expression Regulation, Fungal
Glucose - metabolism
Glycolysis - genetics
Isoenzymes - genetics
Isoenzymes - metabolism
Kluyveromyces - genetics
Kluyveromyces - metabolism
Kluyveromyces lactis
Life Sciences
Monosaccharide Transport Proteins - genetics
Monosaccharide Transport Proteins - metabolism
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Sequence Homology, Amino Acid
Signal Transduction
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Sensing of extracellular glucose is necessary for cells to adapt to glucose variation in their environment. In the respiratory yeast Kluyveromyces lactis , extracellular glucose controls the expression of major glucose permease gene RAG1 through a cascade similar to the Saccharomyces cerevisiae Snf3/Rgt2/Rgt1 glucose signaling pathway. This regulation depends also on intracellular glucose metabolism since we previously showed that glucose induction of the RAG1 gene is abolished in glycolytic mutants. Here we show that glycolysis regulates RAG1 expression through the K. lactis Rgt1 (KlRgt1) glucose signaling pathway by targeting the localization and probably the stability of Rag4, the single Snf3/Rgt2-type glucose sensor of K. lactis . Additionally, the control exerted by glycolysis on glucose signaling seems to be conserved in S. cerevisiae . This retrocontrol might prevent yeasts from unnecessary glucose transport and intracellular glucose accumulation.
ISSN:1098-5549
0270-7306
1098-5549
DOI:10.1128/MCB.00515-14