Effect of activated charcoal on bioremediation of diesel fuel-contaminated soil

• Published in: Microbiology (New York) 2014-09, Vol.83 (5), p.589-598
• Main Authors: Semenyuk, N. N., Yatsenko, V. S., Strijakova, E. R., Filonov, A. E., Petrikov, K. V., Zavgorodnyaya, Yu. A., Vasilyeva, G. K.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Experimental Articles; Life Sciences; Medical Microbiology; Microbiology
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261714050221

Elevated plant and microbial toxicity throughout the season was found in field experiments on bioremediation of diesel fuel (DF)-contaminated soil (4.5%). This was an indication of the presence of mobile toxic DF components and their metabolites. Introduction of granulated activated charcoal (GAC) was shown to decrease the bio- and phytotoxicity of petroleum-contaminated soil, resulting in a sharp increase in the abundance of petroleum degraders (both aboriginal and inoculated ones), as well as to create conditions for improved growth at the stage of post-treatment by phytoremediation. In spite of short-time deceleration of DF degradation, more complete decrease of hydrocarbon concentrations occurred in the presence of GAC, while simultaneous introduction of the sorbent and a biopreparation (and association of mesophilic petroleum-degrading strains) provided the best results. In these variants the concentration of petroleum hydrocarbons decreased to 0.19–0.21 and 0.13–0.14%, respectively, which was 1.5 and 2 times lower than the values for unsupplemented control. Thus, GAC introduction during bioremediation of DF-contaminated soils increases the efficiency of remediation and localizes the pollutants in the treated layer, which decreases the risk of their penetration into groundwater during in situ soil treatment.