Quantitative determination of glycine in commercial dosage forms by kinetic spectrophotometry

A kinetic method for the determination of nanogram quantities of amino acid glycine (Gly) is described. The catalytic activity of cobalt in the reaction of oxidation of purpurin (1,2,4-trihydroxyantraquinone, PP) by hydrogen peroxide in alkaline buffer solution decreases in the presence of micro qua...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2009-07, Vol.64 (7), p.683-689
Main Authors: Mitić, Snežana S., Pavlović, Aleksandra N., Tošić, Snežana B., Arsić, Biljana B., Sunarić, Slavica M.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
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Summary:A kinetic method for the determination of nanogram quantities of amino acid glycine (Gly) is described. The catalytic activity of cobalt in the reaction of oxidation of purpurin (1,2,4-trihydroxyantraquinone, PP) by hydrogen peroxide in alkaline buffer solution decreases in the presence of micro quantities of glycine. Operating conditions for the successful determination of glycine were optimized and yielded a theoretical detection limit of 6.5 ng/mL. Kinetic equations are proposed for the investigated process. The interference effects of certain foreign ions and amino acids upon the reaction rate were studied in order to assess the selectivity of the method. The procedure was successfully applied to the determination of glycine in various pharmaceutical samples. The unique features of this procedure are that the determination can be carried out rapidly at room temperature and analysis time is short. The procedure is simple, inexpensive and efficient for use in the analysis of a large number of samples.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934809070053