Silica nanoparticles induce cardiotoxicity interfering with energetic status and Ca 2+ handling in adult rat cardiomyocytes

• Published in: American journal of physiology. Heart and circulatory physiology 2017-04, Vol.312 (4), p.H645-H661
• Main Authors: Guerrero-Beltrán, Carlos Enrique, Bernal-Ramírez, Judith, Lozano, Omar, Oropeza-Almazán, Yuriana, Castillo, Elena Cristina, Garza, Jesús Roberto, García, Noemí, Vela, Jorge, García-García, Alejandra, Ortega, Eduardo, Torre-Amione, Guillermo, Ornelas-Soto, Nancy, García-Rivas, Gerardo
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; Animals; Calcium - metabolism; Cardiotoxicity - metabolism; Cell Membrane - drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Energy Metabolism - drug effects; Glutathione - metabolism; Male; Membrane Potential, Mitochondrial - drug effects; Mitochondria, Heart - drug effects; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Nanoparticles - toxicity; Oxidative Stress - drug effects; Rats; Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism; Silicon Dioxide - toxicity
• ISSN: 0363-6135; 1522-1539
• DOI: 10.1152/ajpheart.00564.2016

Recent evidence has shown that nanoparticles that have been used to improve or create new functional properties for common products may pose potential risks to human health. Silicon dioxide (SiO ) has emerged as a promising therapy vector for the heart. However, its potential toxicity and mechanisms of damage remain poorly understood. This study provides the first exploration of SiO -induced toxicity in cultured cardiomyocytes exposed to 7- or 670-nm SiO particles. We evaluated the mechanism of cell death in isolated adult cardiomyocytes exposed to 24-h incubation. The SiO cell membrane association and internalization were analyzed. SiO showed a dose-dependent cytotoxic effect with a half-maximal inhibitory concentration for the 7 nm (99.5 ± 12.4 µg/ml) and 670 nm (>1,500 µg/ml) particles, which indicates size-dependent toxicity. We evaluated cardiomyocyte shortening and intracellular Ca handling, which showed impaired contractility and intracellular Ca transient amplitude during β-adrenergic stimulation in SiO treatment. The time to 50% Ca decay increased 39%, and the Ca spark frequency and amplitude decreased by 35 and 21%, respectively, which suggest a reduction in sarcoplasmic reticulum Ca -ATPase (SERCA) activity. Moreover, SiO treatment depolarized the mitochondrial membrane potential and decreased ATP production by 55%. Notable glutathione depletion and H O generation were also observed. These data indicate that SiO increases oxidative stress, which leads to mitochondrial dysfunction and low energy status; these underlie reduced SERCA activity, shortened Ca release, and reduced cell shortening. This mechanism of SiO cardiotoxicity potentially plays an important role in the pathophysiology mechanism of heart failure, arrhythmias, and sudden death. Silica particles are used as novel nanotechnology-based vehicles for diagnostics and therapeutics for the heart. However, their potential hazardous effects remain unknown. Here, the cardiotoxicity of silica nanoparticles in rat myocytes has been described for the first time, showing an impairment of mitochondrial function that interfered directly with Ca handling.