Surfactant protein A modulates the differentiation of murine bone marrow-derived dendritic cells

Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710 Surfactant protein A (SP-A) is an innate immune molecule that regulates pathogen clearance and lung inflammation. SP-A modulates innate immune functions such as phagocytosis, cytokine production, and chemotaxis;...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2003-01, Vol.284 (1), p.232-L241
Main Authors: Brinker, Karen G, Garner, Hollie, Wright, Jo Rae
Format: Article
Language:English
Subjects:
Animals
Biomarkers - analysis
Bone Marrow Cells - cytology
Cell Differentiation - drug effects
Cells, Cultured
Cellular Senescence - physiology
Chemotaxis - drug effects
Complement C1q - pharmacology
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - physiology
Endocytosis - drug effects
Female
Male
Mice
Mice, Inbred C57BL
Phenotype
Pulmonary Surfactant-Associated Protein A - pharmacology
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Summary:Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710 Surfactant protein A (SP-A) is an innate immune molecule that regulates pathogen clearance and lung inflammation. SP-A modulates innate immune functions such as phagocytosis, cytokine production, and chemotaxis; however, little is known about regulation of adaptive immunity by SP-A. Dendritic cells (DCs) are the most potent antigen-presenting cell with the unique capacity to activate naïve T cells and initiate adaptive immunity. The goal of this study was to test the hypothesis that SP-A regulates the differentiation of immature DCs into potent T cell stimulators. The data show that incubation of immature DCs for 24 h with SP-A inhibits basal- and LPS-mediated expression of major histocompatibility complex class II and CD86. Stimulation of immature DCs by SP-A also inhibits the allostimulation of T cells, enhances dextran endocytosis, and alters DC chemotaxis toward RANTES and secondary lymphoid tissue chemokine. The effects on DC phenotype and function are similar for the structurally homologous C1q, but not for SP-D. These studies demonstrate that SP-A participates in the adaptive immune response by modulating important immune functions of DCs. maturation; pattern recognition molecules; innate immunity; adaptive immunity
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00187.2002