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Expression of C-type natriuretic peptide during development of rat lung
1 Division of Environmental Medicine, National Defense Medical College Research Institute, and Departments of 4 Pathology and 5 Biochemistry, National Defense Medical College, Tokorozawa 359-8513; 2 Biochemical Division, Aeromedical Laboratory, Japan Air Self-Defense Force, Tachikawa 190-0003; a...
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Published in: | American journal of physiology. Lung cellular and molecular physiology 1999-11, Vol.277 (5), p.996-L1002 |
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Main Authors: | , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | 1 Division of Environmental
Medicine, National Defense Medical College Research Institute, and
Departments of 4 Pathology and
5 Biochemistry, National Defense
Medical College, Tokorozawa 359-8513;
2 Biochemical Division,
Aeromedical Laboratory, Japan Air Self-Defense Force, Tachikawa
190-0003; and 3 Department of
Medicine and Clinical Science, Kyoto University Graduate School of
Medicine, Kyoto 606-8507, Japan
C-type
natriuretic peptide (CNP), recently found to be secreted from vascular
endothelial cells, is now viewed as a novel endothelium-derived
relaxing peptide. However, the distribution and expression of CNP
during cardiopulmonary development is unclear. To follow changes in the
expression of CNP during lung development, we examined rat embryos and
neonates using Northern blot analysis and in situ hybridization for CNP
mRNA and radioimmunoassay and immunohistochemistry for CNP protein. A
substantial expression of CNP mRNA was first detected on postnatal
day 2 , and it thereafter remained
fairly steady. The level of CNP protein also increased rapidly after
postnatal day 1 , reaching a settled
level on postnatal day 4 . CNP protein
and mRNA were detected in the endothelium and smooth muscle cells of
blood vessels and in bronchial airway and alveolar epithelia.
Immunoreactivity for CNP protein in the endothelium of blood vessels
increased to an intense level after the saccular stage. These results
suggest that the changes in CNP levels may be related to the occurrence
of pulmonary vasodilation after birth.
cardiopulmonary system |
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ISSN: | 1040-0605 1522-1504 |
DOI: | 10.1152/ajplung.1999.277.5.l996 |