Autocrine cytokine signaling mediates effects of rhinovirus on airway responsiveness

• Published in: American journal of physiology. Lung cellular and molecular physiology 2000-06, Vol.278 (6), p.1146-L1153
• Main Authors: Grunstein, Michael M, Hakonarson, Hakon, Maskeri, Neil, Chuang, Sing
• Format: Article
• Language: English
• Subjects: Animals; Autocrine Communication - physiology; Cytokines - physiology; Gene Expression Regulation, Viral - physiology; Humans; Interleukin-1 - metabolism; Interleukin-1 - physiology; Interleukin-5 - genetics; Interleukin-5 - physiology; Muscle, Smooth - physiopathology; Muscle, Smooth - virology; Rabbits; Rhinovirus - physiology; Trachea - physiopathology; Trachea - virology
• ISSN: 1040-0605; 1522-1504
• DOI: 10.1152/ajplung.2000.278.6.l1146

Division of Pulmonary Medicine, Joseph Stokes, Jr. Research Institute, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 The airway responses to allergen exposure in allergic asthma are qualitatively similar to those elicited by specific viral respiratory pathogens, most notably rhinovirus (RV), suggesting that the altered airway responsiveness seen in allergic asthma and that elicited by viral respiratory tract infection may share a common underlying mechanism. To the extent that T helper cell type 2 (Th2) cytokines have been implicated in the pathogenesis of allergic asthma, this study examined the potential role(s) of Th2-type cytokines in mediating pro-asthmatic-like changes in airway smooth muscle (ASM) responsiveness after inoculation of naive ASM with human RV. Isolated rabbit ASM tissues and cultured human ASM cells were exposed to RV (serotype 16) for 24 h in the absence and presence of monoclonal blocking antibodies (MAbs) or antagonists directed against either the Th2-type cytokines interleukin (IL)-4 and IL-5, intercellular adhesion molecule (ICAM)-1 (the endogenous host receptor for most RVs), or the pleiotropic proinflammatory cytokine IL-1 . Relative to control (vehicle-treated) tissues, RV-exposed ASM exhibited significantly enhanced isometric contractility to acetylcholine and impaired relaxation to isoproterenol. These pro-asthmatic-like changes in ASM responsiveness were ablated by pretreating the RV-exposed tissues with either IL-5-receptor- blocking antibody or human recombinant IL-1-receptor antagonist, whereas IL-4 neutralizing antibody had no effect. Extended studies further demonstrated that inoculation of ASM cells with RV elicited 1 ) an increased mRNA expression and release of IL-5 protein, which was inhibited in the presence of anti-ICAM-1 MAb, and 2 ) an enhanced release of IL-1 protein, which was inhibited in the presence of IL-5 receptor- antibody. Collectively, these observations provide new evidence demonstrating that RV-induced changes in ASM responsiveness are largely attributed to ICAM-1-dependent activation of a cooperative autocrine signaling mechanism involving upregulated IL-5-mediated release of IL-1 by the RV-exposed ASM itself. T helper cell type 2 cytokines; airway smooth muscle; asthma