Monoaminergic Establishment of Rostrocaudal Gradients of Rhythmicity in the Neonatal Mouse Spinal Cord

• Published in: Journal of neurophysiology 2005-08, Vol.94 (2), p.1554-1564
• Main Authors: Christie, Kimberly J, Whelan, Patrick J
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn - physiology; Benzazepines - pharmacology; Biogenic Monoamines - physiology; Dopamine - pharmacology; Dopamine Agonists - pharmacology; Dopamine Antagonists - pharmacology; Dose-Response Relationship, Drug; Drug Combinations; Evoked Potentials - drug effects; Evoked Potentials - physiology; Excitatory Amino Acid Agonists - pharmacology; Fluorescent Dyes - metabolism; Functional Laterality; GABA Antagonists - pharmacology; Glycine Agents - pharmacology; In Vitro Techniques; Lumbosacral Region; Mice; N-Methylaspartate - pharmacology; Nerve Net - physiology; Neural Networks (Computer); Organic Chemicals; Periodicity; Picrotoxin - pharmacology; Quinpirole - pharmacology; Serotonin - pharmacology; Spinal Cord - drug effects; Spinal Cord - physiology; Spinal Cord Injuries; Strychnine - pharmacology
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.00299.2005

1 Hotchkiss Brain Institute, 2 Department of Physiology and Biophysics, and 3 Department of Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada Submitted 21 March 2005; accepted in final form 11 April 2005 Bath application of monoamines is a potent method for evoking locomotor activity in neonatal rats and mice. Monoamines also promote functional recovery in adult animals with spinal cord injuries by activating spinal cord networks. However, the mechanisms of their actions on spinal networks are largely unknown. In this study, we tested the hypothesis that monoamines establish rostrocaudal gradients of rhythmicity in the thoracolumbar spinal cord. Isolated neonatal mouse spinal cord preparations (P0–P2) were used. To assay excitability of networks by monoamines, we evoked a disinhibited rhythm by bath application of picrotoxin and strychnine and recorded neurograms from several thoracolumbar ventral roots. We first established that rostral and caudal segments of the thoracolumbar spinal cord had equal excitability by completely transecting preparations at the L 3 segmental level and recording the frequency of the disinhibited rhythm from both segments. Next we established that a majority of ventral interneurons retrogradely labeled by calcium green dextran were active during network activity. We then bath applied combinations of monoaminergic agonists [5-HT and dopamine (DA)] known to elicit locomotor activity. Our results show that monoamines establish rostrocaudal gradients of rhythmicity in the thoracolumbar spinal cord. This may be one mechanism by which combinations of monoaminergic compounds normally stably activate locomotor networks. Address for reprint requests and other correspondence: P. J. Whelan, HSC 2119, Dept. of Physiology and Biophysics, Univ. of Calgary, Calgary, AB T2N 4N1, Canada (E-mail: whelan{at}ucalgary.ca )