Abstract 2192: Functional genomic screen to identify candidates capable of modulating invasive properties of HNSCC

• Published in: Cancer research (Chicago, Ill.) Ill.), 2010-04, Vol.70 (8_Supplement), p.2192-2192
• Main Authors: Lepikhova, Tatiana N., Imai, Misa, Peitsaro, Nina, Klefstrom, Juha, Monni, Outi M.
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM10-2192

Head and neck squamous cell carcinomas (HNSCC) contain a heterogeneous population of cancer cells some of which is explained by ongoing mutations that occur due to genetic instability and environmental factors. Not all of the cancer cells have a similar ability to drive tumor formation. To identify genes capable of modulating invasive properties in head and neck squamous cell carcinoma (HNSCC), we have compared copy number profiles (244K aCGH) of 12 primary and metastatic cancer cell lines obtained from patients with HNSCC of tongue and larynx. A specific pattern of copy number gains and losses was present in each cell line studied. Majority of the changes occurred in both the primary tumor and corresponding metastasis, but some amplifications, such as 10q21.1., 11q22, and 17p12 occurred more frequently in metastatic cancers. To determine whether any genes located at the amplicons input to metastatic potential of HNSCC we are performing a systematic loss-of-function survey. First, we examined the cell growth and invasive properties of primary and metastatic cell lines in vitro and in vivo. We then set up a genetic screen using highly metastatic cell lines in in vitro invasion assay system. pLKO1 shRNA library targeting genes located at identified amplified regions in invasive cells were transduced into the selected cell lines and transduced cell lines were subjected to transwell invasion assay. Several genes targeted for amplification and capable of modulating invasive properties of HNSCC in vitro have been selected for further validation in vivo. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2192.