Abstract 777: Rosehip (Rosa canina) extracts prevent Akt-mediated cell proliferation in glioblastoma cells

• Published in: Cancer research (Chicago, Ill.) Ill.), 2010-04, Vol.70 (8_Supplement), p.777-777
• Main Authors: McNair, Patrice, Goktepe, Ipek, Martin, Patrick M.
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM10-777

Glioblastoma multiforme (GBM) are aggressive malignant tumors that develop in the brain. GBMs demonstrate increased rates of cell proliferation, a lack of apoptosis as well the propensity to migrate to distal sites in the brain. The clinical management of glioblastoma consists of surgical resection followed by radiotherapy and chemotherapy. However, this treatment regimen is not very effective and more effective therapies need to be developed. Activation of several signaling pathways has been associated with the increased proliferative capacity of GBM cells. Activity of the Akt/mTOR pathway is exceptionally high in GBMs and may regulate sensitivity to chemotherapy and radiotherapy. Akt and its downstream effector p70S6 kinase (p70S6K) demonstrate a high amount of activity in GBMs. Inactivation of Akt and p70S6K attenuate GBM cell growth and may inhibit GBM cell proliferation. Therefore, we investigated the antiproliferative capacity of extracts from the Rosehip (Rosa canina) plant. Rosehip extracts have been used as dietary supplements to relieve symptoms associated with diarrhea, gastritis and rheumatoid arthritis. U-1242MG, a human GBM cell line, treated with Rosehip extracts (250 µg/ml) demonstrated a decrease in cell proliferation. The Rosehip extract-mediated decrease in cell proliferation was equal to the decrease of cell proliferation observed when LY294002 (20 µM),a known Akt inhibitor was utilized. Additionally, pretreatment of the U-1242 MG cells with these Rosehip extracts (250 µg/ml) decreased p70S6K phosphorylation, suggesting these extracts prevent GBM cell proliferation by blocking Akt/mTOR signaling mechanisms. Taken together these data suggest that p70S6K positively regulates GBM cell proliferation, and Rosehip extracts may serve as an alternative or supplement to current therapeutic regimens for GBMs. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 777.