Abstract 2800: Dietary black raspberries (BRBs) inhibit tumor progression in PTEN-deficient mouse model of prostate cancer

• Published in: Cancer research (Chicago, Ill.) Ill.), 2015-08, Vol.75 (15_Supplement), p.2800-2800
• Main Authors: Narayanan, Narayanan K., Stoner, Gary D., Peiffer, Daniel S., Galdanes, Karen, Larios, Eric, Mark, Alu, Maziniski, Lisa, Chiriboga, Luis, Bosland, Maarten C.
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2015-2800

Black raspberries (BRBs) a natural food demonstrated to have anti-oxidant, anti-inflammatory and anti-cancer activities,have been shown to inhibit oral, esophageal, mammary gland and colon cancers in rodents. Several human trials have been completed to date to assess the efficacy of BRB formulations for cancer prevention. However, the chemopreventive potential of BRBs against prostate cancer, the most commonly diagnosed malignancy and the second leading cause of death among men in the United States, is yet to be reported. Among the murine models, PTEN-mutant mice develop tumors in situ that faithfully mimic the intratumor heterogeneity observed during the progression of human prostate cancer. Therefore, we utilized this clinically relevant PTEN-mutant mouse model to evaluate the chemopreventive potential of BRBs against prostate cancer. Genotyped 5-week-old male PTEN-mutant mice, randomly assigned to control and treatment groups (n = 12 mice/group), were fed AIN-93G diet (control) or AIN-93G diet supplemented with BRBs (5 or 10%) for 23 weeks. Age-matched non-transgenic mice (wild-type) served as experimental controls (n = 6). Animal weight and food consumption were measured during the treatment period, and the mice were euthanized at 28 weeks of age. Prostate tissues were harvested, weighed and fixed in 10% formalin for histopathological analysis. Histological, cell proliferation (Ki-67 staining) and apoptosis (TUNEL) analyses were performed to determine the chemopreventive potential of BRBs. PTEN-mutant mice fed control or BRB (5 or 10%) diets had steady body weight gain, 16 to 18 g during the 23-week treatment. BRB diets were well tolerated as none of the animals fed BRBs exhibited any observable toxicity. PTEN-mutant mice (control) had increased prostate weight (ave. = 248 mg) relative to wild-type mouse prostate (ave. = 90 mg), and 5 and 10% BRB diets significantly (p