Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development

• Published in: Medical principles and practice 2024-04, Vol.33 (2), p.112-121
• Main Authors: Gödöny, Krisztina, Herczeg, Róbert, Gyenesei, Attila, Várnagy, Ákos, Bognár, Zoltán, Kovács, Gábor L., Szekeres-Barthó, Júlia, Mauchart, Péter, Nagy, Bernadett, Erostyák, János, Kovács, Kálmán, Bódis, József
• Format: Article
• Language: English
• Subjects: Aluminum; Cell division; DNA damage; DNA repair; Embryos; Females; In vitro fertilization; Infertility; Laboratories; Original Paper; Oxidation; Physiology; Reproductive technologies; Sperm; Success
• ISSN: 1011-7571; 1423-0151; 1423-0151
• DOI: 10.1159/000536358

Abstract Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety. Highlights of the StudyWe compared the results of IVF between two experimental setups: light protection with aluminum foil and red filters, and no light protection as a control.Conventional embryo culture conditions were improved by using special light filters for light protection.Reducing the embryotoxic wavelengths of light in IVF centers improved the development rate of embryos and also embryo quality, while maintaining embryo safety.