Structural and Functional Characterization of a Na v 1.5-Mitochondrial Couplon

• Published in: Circulation research 2021-02, Vol.128 (3), p.419-432
• Main Authors: Pérez-Hernández, Marta, Leo-Macias, Alejandra, Keegan, Sarah, Jouni, Mariam, Kim, Joon-Chul, Agullo-Pascual, Esperanza, Vermij, Sarah, Zhang, Mingliang, Liang, Feng-Xia, Burridge, Paul, Fenyo, David, Rothenberg, Eli, Delmar, Mario
• Format: Article
• Language: English
• Subjects: Animals; Calcium - metabolism; Calcium Signaling; Cell Line; Female; Humans; Kinetics; Male; Mice; Mice, Inbred C57BL; Microscopy, Electron, Scanning; Mitochondria, Heart - metabolism; Mitochondria, Heart - ultrastructure; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - ultrastructure; NAV1.5 Voltage-Gated Sodium Channel - genetics; NAV1.5 Voltage-Gated Sodium Channel - metabolism; Single Molecule Imaging; Sodium-Calcium Exchanger - genetics; Sodium-Calcium Exchanger - metabolism; Superoxides - metabolism
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/CIRCRESAHA.120.318239

The cardiac sodium channel Na 1.5 has a fundamental role in excitability and conduction. Previous studies have shown that sodium channels cluster together in specific cellular subdomains. Their association with intracellular organelles in defined regions of the myocytes, and the functional consequences of that association, remain to be defined. To characterize a subcellular domain formed by sodium channel clusters in the crest region of the myocytes and the subjacent subsarcolemmal mitochondria. Through a combination of imaging approaches including super-resolution microscopy and electron microscopy we identified, in adult cardiac myocytes, a Na 1.5 subpopulation in close proximity to subjacent subsarcolemmal mitochondria; we further found that subjacent subsarcolemmal mitochondria preferentially host the mitochondrial NCLX (Na /Ca exchanger). This anatomic proximity led us to investigate functional changes in mitochondria resulting from sodium channel activity. Upon TTX (tetrodotoxin) exposure, mitochondria near Na 1.5 channels accumulated more Ca and showed increased reactive oxygen species production when compared with interfibrillar mitochondria. Finally, crosstalk between Na 1.5 channels and mitochondria was analyzed at a transcriptional level. We found that (encoding Na 1.5) and (which encode Na 1.5 and NCLX, respectively) are negatively correlated both in a human transcriptome data set (Genotype-Tissue Expression) and in human-induced pluripotent stem cell-derived cardiac myocytes deficient in . We describe an anatomic hub (a couplon) formed by sodium channel clusters and subjacent subsarcolemmal mitochondria. Preferential localization of NCLX to this domain allows for functional coupling where the extrusion of Ca from the mitochondria is powered, at least in part, by the entry of sodium through Na 1.5 channels. These results provide a novel entry-point into a mechanistic understanding of the intersection between electrical and structural functions of the heart.