Abstract 19234: A Short Term Premorbid High Fat Diet Disrupts Cardiac Leptin Signaling, Impairs Glucose Metabolism and Increases Morbidity and Mortality with Myocardial Infarction

• Published in: Circulation (New York, N.Y.) N.Y.), 2012-11, Vol.126 (suppl_21)
• Main Authors: McGaffin, Kenneth, Yester, Keith, Witham, William
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.126.suppl_21.A19234

Abstract only Introduction A habitual high fat diet (HFD) contributes to obesity, and increases morbidity in cardiovascular disease (CVD), whereas the effects of a short-term HFD on CVD outcomes are unknown. This study was performed to test the hypothesis that a short term premorbid HFD disrupts cardiac leptin signaling, impairs glucose metabolism and exacerbates morbidity and mortality with myocardial infarction (MI). Methods/Results Lean, 12 week old male C57BL/6 mice were provided either a HFD (60% fat calories; n=24) or regular chow (RC) (16% fat calories; n=24) for 7 days. Equal number of mice from each dietary group were then randomly assigned to experimental MI or sham procedure, and maintained on RC until study completion at 7 days. Echocardiography and cardiac catheterization were performed on all surviving mice, and hearts were examined for biochemical and histologic measures of cardiac injury, and oxidative metabolism. Results are presented as mean ± SEM with statistical comparisons by t-test, or log-rank Mantel-Cox test (for survival). Groups of sham mice were not significantly different from each other in any measured outcome. However, with MI, a HFD increased mortality (41 vs 19%), cardiac dysfunction (fractional shortening 23±1 vs 29±1% and ejection fraction 24±2 vs 36±2%), and cardiac dilation (end diastolic dimension 4.6±0.1 vs 3.9±0.1mm and volume 56.6±1.3 vs 44.3±2.5uL); all p