Abstract 17122: Human iPS Cell-derived 3D Engineered Cardiac Tissue Including Cardiomyocytes and Multiple Vascular Lineages for Cardiac Regeneration

• Published in: Circulation (New York, N.Y.) N.Y.), 2015-11, Vol.132 (Suppl_3 Suppl 3), p.A17122-A17122
• Main Authors: Masumoto, Hidetoshi, Nakane, Takeichiro, Tinney, Joseph P, Yuan, Fangping, Ye, Fei, Kowalski, William J, Sakata, Ryuzo, Yamashita, Jun K, Keller, Bradley B
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.132.suppl_3.17122

BackgroundThree-dimensional (3D) engineered cardiac tissues (ECTs) are a robust platform technology to investigate cardiovascular (CV) cell function and provide an excellent microenvironment for tissue implantation and cardiac repair. We generated 3D ECTs using cardiomyocytes (CMs), endothelial cells (ECs), and vascular mural cells (MCs) efficiently differentiated from human iPS cells (hiPSCs).Methods & ResultsWe employed 3 different monolayer culture-based differentiation protocols1) CM+EC protocolmesoderm induction followed by VEGF (mes+VEGF) produced a distribution of 61.8±8.0% cTnT-CMs, 19.4±9.1% VE-cadherin-ECs, and 1.7±2.0% PDGFRβ-MCs, (n=26, day15); 2) CM+MC protocolmes+Dkk1 to induce CMs and MCs; and 3) MC protocolexclusive induction of MCs. We collected the cells on differentiation day 15 and mixed them to generate 3 classes of ECTs composed of different CV cell population patterns1). CM+EC; 2). CM+MC, and 3). CM+EC+MC. We seeded the each cell mixture in a collagen/Matrigel mixture to form spontaneously beating ECTs. In vitro force measurement analysis showed that CM+EC+MC ECTs possessed the highest maximum capture rate (4.6±0.6 Hz, P