Abstract 24077: Differential, Sex Related, Response To High-fat Diet In Mice Impairs Cardiac Autophagy And Mitophagy

• Published in: Circulation (New York, N.Y.) N.Y.), 2017-11, Vol.136 (suppl_1)
• Main Authors: Thomas, Amandine, Marek, Stefanie, Tucker, Kyle C, Andres, Allen M, Gottlieb, Roberta A
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.136.suppl_1.24077

Abstract only Introduction: Premenopausal women, as well as females in animal studies, have a reduced risk of cardiovascular diseases and a reduced myocardial susceptibility to ischemia/reperfusion (I/R) injury. However, with constantly increasing prevalence of obesity, the impact of diet-induced obesity on females remains unclear. Mechanisms of autophagy and mitophagy, differentially regulated between males and females, have been shown to be cardioprotective and impacted under nutritional overload. Hypothesis: Autophagy and mitophagy pathways can be involved in both female cardio protection and deleterious effect of obesity. Methods: Male and female C57Bl/6J mice (n =20 per group) of 8 weeks old were fed a low-fat (LFD, 10% fat) or high-fat (HFD, 60% fat) diet for 12 weeks. At 12 weeks, hearts from mice were studied under basal conditions and after global no-flow ischemia and reperfusion on a Langendorff perfusion system. Results: In both males and females, HFD significantly increases body weight (31.4g vs 48.3g and 22.1g vs 39.9g respectively), fat mass (+200% in males and +300% in females) and blood glucose (+79mg/dl and + 54mg/dl, respectively) (all p